The m(6)A-methylase complex recruits TREX and regulates mRNA export

N(6)-methyladenosine (m(6)A) is the most abundant internal modification of eukaryotic mRNA. This modification has previously been shown to alter the export kinetics for mRNAs though the molecular details surrounding this phenomenon remain poorly understood. Recruitment of the TREX mRNA export comple...

Descripción completa

Detalles Bibliográficos
Autores principales: Lesbirel, Simon, Viphakone, Nicolas, Parker, Matthew, Parker, Jacob, Heath, Catherine, Sudbery, Ian, Wilson, Stuart A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6138711/
https://www.ncbi.nlm.nih.gov/pubmed/30218090
http://dx.doi.org/10.1038/s41598-018-32310-8
_version_ 1783355383783358464
author Lesbirel, Simon
Viphakone, Nicolas
Parker, Matthew
Parker, Jacob
Heath, Catherine
Sudbery, Ian
Wilson, Stuart A.
author_facet Lesbirel, Simon
Viphakone, Nicolas
Parker, Matthew
Parker, Jacob
Heath, Catherine
Sudbery, Ian
Wilson, Stuart A.
author_sort Lesbirel, Simon
collection PubMed
description N(6)-methyladenosine (m(6)A) is the most abundant internal modification of eukaryotic mRNA. This modification has previously been shown to alter the export kinetics for mRNAs though the molecular details surrounding this phenomenon remain poorly understood. Recruitment of the TREX mRNA export complex to mRNA is driven by transcription, 5′ capping and pre-mRNA splicing. Here we identify a fourth mechanism in human cells driving the association of TREX with mRNA involving the m(6)A methylase complex. We show that the m(6)A complex recruits TREX to m(6)A modified mRNAs and this process is essential for their efficient export. TREX also stimulates recruitment of the m(6)A reader protein YTHDC1 to the mRNA and the m(6)A complex influences the interaction of TREX with YTHDC1. Together our studies reveal a key role for TREX in the export of m(6)A modified mRNAs.
format Online
Article
Text
id pubmed-6138711
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-61387112018-09-15 The m(6)A-methylase complex recruits TREX and regulates mRNA export Lesbirel, Simon Viphakone, Nicolas Parker, Matthew Parker, Jacob Heath, Catherine Sudbery, Ian Wilson, Stuart A. Sci Rep Article N(6)-methyladenosine (m(6)A) is the most abundant internal modification of eukaryotic mRNA. This modification has previously been shown to alter the export kinetics for mRNAs though the molecular details surrounding this phenomenon remain poorly understood. Recruitment of the TREX mRNA export complex to mRNA is driven by transcription, 5′ capping and pre-mRNA splicing. Here we identify a fourth mechanism in human cells driving the association of TREX with mRNA involving the m(6)A methylase complex. We show that the m(6)A complex recruits TREX to m(6)A modified mRNAs and this process is essential for their efficient export. TREX also stimulates recruitment of the m(6)A reader protein YTHDC1 to the mRNA and the m(6)A complex influences the interaction of TREX with YTHDC1. Together our studies reveal a key role for TREX in the export of m(6)A modified mRNAs. Nature Publishing Group UK 2018-09-14 /pmc/articles/PMC6138711/ /pubmed/30218090 http://dx.doi.org/10.1038/s41598-018-32310-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lesbirel, Simon
Viphakone, Nicolas
Parker, Matthew
Parker, Jacob
Heath, Catherine
Sudbery, Ian
Wilson, Stuart A.
The m(6)A-methylase complex recruits TREX and regulates mRNA export
title The m(6)A-methylase complex recruits TREX and regulates mRNA export
title_full The m(6)A-methylase complex recruits TREX and regulates mRNA export
title_fullStr The m(6)A-methylase complex recruits TREX and regulates mRNA export
title_full_unstemmed The m(6)A-methylase complex recruits TREX and regulates mRNA export
title_short The m(6)A-methylase complex recruits TREX and regulates mRNA export
title_sort m(6)a-methylase complex recruits trex and regulates mrna export
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6138711/
https://www.ncbi.nlm.nih.gov/pubmed/30218090
http://dx.doi.org/10.1038/s41598-018-32310-8
work_keys_str_mv AT lesbirelsimon them6amethylasecomplexrecruitstrexandregulatesmrnaexport
AT viphakonenicolas them6amethylasecomplexrecruitstrexandregulatesmrnaexport
AT parkermatthew them6amethylasecomplexrecruitstrexandregulatesmrnaexport
AT parkerjacob them6amethylasecomplexrecruitstrexandregulatesmrnaexport
AT heathcatherine them6amethylasecomplexrecruitstrexandregulatesmrnaexport
AT sudberyian them6amethylasecomplexrecruitstrexandregulatesmrnaexport
AT wilsonstuarta them6amethylasecomplexrecruitstrexandregulatesmrnaexport
AT lesbirelsimon m6amethylasecomplexrecruitstrexandregulatesmrnaexport
AT viphakonenicolas m6amethylasecomplexrecruitstrexandregulatesmrnaexport
AT parkermatthew m6amethylasecomplexrecruitstrexandregulatesmrnaexport
AT parkerjacob m6amethylasecomplexrecruitstrexandregulatesmrnaexport
AT heathcatherine m6amethylasecomplexrecruitstrexandregulatesmrnaexport
AT sudberyian m6amethylasecomplexrecruitstrexandregulatesmrnaexport
AT wilsonstuarta m6amethylasecomplexrecruitstrexandregulatesmrnaexport

Ejemplares similares