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Restriction enzyme digestion of host DNA enhances universal detection of parasitic pathogens in blood via targeted amplicon deep sequencing
BACKGROUND: Targeted amplicon deep sequencing (TADS) of the 16S rRNA gene is commonly used to explore and characterize bacterial microbiomes. Meanwhile, attempts to apply TADS to the detection and characterization of entire parasitic communities have been hampered since conserved regions of many con...
Autores principales: | Flaherty, Briana R., Talundzic, Eldin, Barratt, Joel, Kines, Kristine J., Olsen, Christian, Lane, Meredith, Sheth, Mili, Bradbury, Richard S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6142370/ https://www.ncbi.nlm.nih.gov/pubmed/30223888 http://dx.doi.org/10.1186/s40168-018-0540-2 |
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