Continuous directed evolution of proteins with improved soluble expression

We report the development of soluble expression phage-assisted continuous evolution (SE-PACE), a system for rapidly evolving proteins with increased soluble expression. Through use of a PACE-compatible AND gate that uses a split-intein pIII, SE-PACE enables two simultaneous positive selections to ev...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Tina, Badran, Ahmed H., Huang, Tony P., Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143403/
https://www.ncbi.nlm.nih.gov/pubmed/30127387
http://dx.doi.org/10.1038/s41589-018-0121-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143403/
https://www.ncbi.nlm.nih.gov/pubmed/30127387
http://dx.doi.org/10.1038/s41589-018-0121-5

Ejemplares similares