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Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance

Stem and progenitor cells of the submandibular salivary gland (SMG) give rise to, maintain, and regenerate the multiple lineages of mature epithelial cells including those belonging to the ductal, acinar, basal and myoepithelial subtypes. Here we have exploited single cell RNA-sequencing and in vivo...

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Autores principales: Song, Eun-Ah Christine, Min, Sangwon, Oyelakin, Akinsola, Smalley, Kirsten, Bard, Jonathan E., Liao, Lan, Xu, Jianming, Romano, Rose-Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6145895/
https://www.ncbi.nlm.nih.gov/pubmed/30232460
http://dx.doi.org/10.1038/s41598-018-32343-z
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author Song, Eun-Ah Christine
Min, Sangwon
Oyelakin, Akinsola
Smalley, Kirsten
Bard, Jonathan E.
Liao, Lan
Xu, Jianming
Romano, Rose-Anne
author_facet Song, Eun-Ah Christine
Min, Sangwon
Oyelakin, Akinsola
Smalley, Kirsten
Bard, Jonathan E.
Liao, Lan
Xu, Jianming
Romano, Rose-Anne
author_sort Song, Eun-Ah Christine
collection PubMed
description Stem and progenitor cells of the submandibular salivary gland (SMG) give rise to, maintain, and regenerate the multiple lineages of mature epithelial cells including those belonging to the ductal, acinar, basal and myoepithelial subtypes. Here we have exploited single cell RNA-sequencing and in vivo genetic lineage tracing technologies to generate a detailed map of the cell fate trajectories and branch points of the basal and myoepithelial cell populations of the mouse SMG during embryonic development and in adults. Our studies show that the transcription factor p63 and alpha-smooth muscle actin (SMA) serve as faithful markers of the basal and myoepithelial cell lineages, respectively and that both cell types are endowed with progenitor cell properties. However, p63(+) basal and SMA(+) myoepithelial cells exhibit distinct cell fates by virtue of maintaining different cellular lineages during morphogenesis and in adults. Collectively, our results reveal the dynamic and complex nature of the diverse SMG cell populations and highlight the distinct differentiation potential of the p63 and SMA expressing subtypes in the stem and progenitor cell hierarchy. Long term these findings have profound implications towards a better understanding of the molecular mechanisms that dictate lineage commitment and differentiation programs during development and adult gland maintenance.
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spelling pubmed-61458952018-09-24 Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance Song, Eun-Ah Christine Min, Sangwon Oyelakin, Akinsola Smalley, Kirsten Bard, Jonathan E. Liao, Lan Xu, Jianming Romano, Rose-Anne Sci Rep Article Stem and progenitor cells of the submandibular salivary gland (SMG) give rise to, maintain, and regenerate the multiple lineages of mature epithelial cells including those belonging to the ductal, acinar, basal and myoepithelial subtypes. Here we have exploited single cell RNA-sequencing and in vivo genetic lineage tracing technologies to generate a detailed map of the cell fate trajectories and branch points of the basal and myoepithelial cell populations of the mouse SMG during embryonic development and in adults. Our studies show that the transcription factor p63 and alpha-smooth muscle actin (SMA) serve as faithful markers of the basal and myoepithelial cell lineages, respectively and that both cell types are endowed with progenitor cell properties. However, p63(+) basal and SMA(+) myoepithelial cells exhibit distinct cell fates by virtue of maintaining different cellular lineages during morphogenesis and in adults. Collectively, our results reveal the dynamic and complex nature of the diverse SMG cell populations and highlight the distinct differentiation potential of the p63 and SMA expressing subtypes in the stem and progenitor cell hierarchy. Long term these findings have profound implications towards a better understanding of the molecular mechanisms that dictate lineage commitment and differentiation programs during development and adult gland maintenance. Nature Publishing Group UK 2018-09-19 /pmc/articles/PMC6145895/ /pubmed/30232460 http://dx.doi.org/10.1038/s41598-018-32343-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Song, Eun-Ah Christine
Min, Sangwon
Oyelakin, Akinsola
Smalley, Kirsten
Bard, Jonathan E.
Liao, Lan
Xu, Jianming
Romano, Rose-Anne
Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance
title Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance
title_full Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance
title_fullStr Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance
title_full_unstemmed Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance
title_short Genetic and scRNA-seq Analysis Reveals Distinct Cell Populations that Contribute to Salivary Gland Development and Maintenance
title_sort genetic and scrna-seq analysis reveals distinct cell populations that contribute to salivary gland development and maintenance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6145895/
https://www.ncbi.nlm.nih.gov/pubmed/30232460
http://dx.doi.org/10.1038/s41598-018-32343-z
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