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CRISPR-typing PCR (ctPCR), a new Cas9-based DNA detection method

This study develops a new method for detecting and typing target DNA based on Cas9 nuclease, which was named as ctPCR, representing Cas9-sgRNA- or CRISPR-typing PCR. The technique can detect and type target DNA easily, rapidly, specifically, and sensitively. This technique detects target DNA in thre...

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Detalles Bibliográficos
Autores principales: Wang, Qiao, Zhang, Beibei, Xu, Xinhui, Long, Feifei, Wang, Jinke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6148268/
https://www.ncbi.nlm.nih.gov/pubmed/30237405
http://dx.doi.org/10.1038/s41598-018-32329-x