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CRISPR-typing PCR (ctPCR), a new Cas9-based DNA detection method
This study develops a new method for detecting and typing target DNA based on Cas9 nuclease, which was named as ctPCR, representing Cas9-sgRNA- or CRISPR-typing PCR. The technique can detect and type target DNA easily, rapidly, specifically, and sensitively. This technique detects target DNA in thre...
Autores principales: | Wang, Qiao, Zhang, Beibei, Xu, Xinhui, Long, Feifei, Wang, Jinke |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6148268/ https://www.ncbi.nlm.nih.gov/pubmed/30237405 http://dx.doi.org/10.1038/s41598-018-32329-x |
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