Engineering the l-Arabinose Isomerase from Enterococcus Faecium for d-Tagatose Synthesis

l-Arabinose isomerase (EC 5.3.1.4) (l-AI) from Enterococcus faecium DBFIQ E36 was overproduced in Escherichia coli by designing a codon-optimized synthetic araA gene. Using this optimized gene, two N- and C-terminal His-tagged-l-AI proteins were produced. The cloning of the two chimeric genes into r...

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Detalles Bibliográficos
Autores principales: de Sousa, Marylane, Manzo, Ricardo M., García, José L., Mammarella, Enrique J., Gonçalves, Luciana R. B., Pessela, Benevides C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6149694/
https://www.ncbi.nlm.nih.gov/pubmed/29211024
http://dx.doi.org/10.3390/molecules22122164

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6149694/
https://www.ncbi.nlm.nih.gov/pubmed/29211024
http://dx.doi.org/10.3390/molecules22122164

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