Improving the Catalytic Property of the Glycoside Hydrolase LXYL-P1–2 by Directed Evolution

The glycoside hydrolase LXYL-P1–2 from Lentinula edodes can specifically hydrolyze 7-β-xylosyltaxanes to form 7-β-hydroxyltaxanes for the semi-synthesis of paclitaxel. In order to improve the catalytic properties of the enzyme, we performed error-prone PCR to construct the random mutant library of L...

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Detalles Bibliográficos
Autores principales: Chen, Jing-Jing, Liang, Xiao, Li, Hui-Xian, Chen, Tian-Jiao, Zhu, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6149855/
https://www.ncbi.nlm.nih.gov/pubmed/29207529
http://dx.doi.org/10.3390/molecules22122133

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6149855/
https://www.ncbi.nlm.nih.gov/pubmed/29207529
http://dx.doi.org/10.3390/molecules22122133

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