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Improving the Catalytic Property of the Glycoside Hydrolase LXYL-P1–2 by Directed Evolution

The glycoside hydrolase LXYL-P1–2 from Lentinula edodes can specifically hydrolyze 7-β-xylosyltaxanes to form 7-β-hydroxyltaxanes for the semi-synthesis of paclitaxel. In order to improve the catalytic properties of the enzyme, we performed error-prone PCR to construct the random mutant library of L...

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Detalles Bibliográficos
Autores principales:	Chen, Jing-Jing, Liang, Xiao, Li, Hui-Xian, Chen, Tian-Jiao, Zhu, Ping
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6149855/ https://www.ncbi.nlm.nih.gov/pubmed/29207529 http://dx.doi.org/10.3390/molecules22122133

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