Cargando…
Inhibition of bcl-2 and cox-2 Protein Expression after Local Application of a New Carmustine-Loaded Clinoptilolite-Based Delivery System in a Chemically Induced Skin Cancer Model in Mice
Our research has focused on in vitro and in vivo evaluations of a new Carmustine (BCNU)-loaded clinoptilolite-based delivery system. Two clinoptilolite ionic forms—hydrogen form (HCLI) and sodium form (NaCLI)—were prepared, allowing a loading degree of about 5–6 mg BCNU/g of zeolite matrix due to th...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6150160/ https://www.ncbi.nlm.nih.gov/pubmed/29156646 http://dx.doi.org/10.3390/molecules22112014 |
_version_ | 1783356933396234240 |
---|---|
author | Ghiciuc, Cristina Mihaela Strat, Aurel Lulu Ochiuz, Lacramioara Lupusoru, Catalina Elena Ignat, Maria Vasile, Aurelia Grigorovici, Alexandru Stoleriu, Iulian Solcan, Carmen |
author_facet | Ghiciuc, Cristina Mihaela Strat, Aurel Lulu Ochiuz, Lacramioara Lupusoru, Catalina Elena Ignat, Maria Vasile, Aurelia Grigorovici, Alexandru Stoleriu, Iulian Solcan, Carmen |
author_sort | Ghiciuc, Cristina Mihaela |
collection | PubMed |
description | Our research has focused on in vitro and in vivo evaluations of a new Carmustine (BCNU)-loaded clinoptilolite-based delivery system. Two clinoptilolite ionic forms—hydrogen form (HCLI) and sodium form (NaCLI)—were prepared, allowing a loading degree of about 5–6 mg BCNU/g of zeolite matrix due to the dual porous feature of clinoptilolite. Clinoptilolite-based delivery systems released 35.23% of the load in 12 h for the BCNU@HCLI system and only 10.82% for the BCNU@NaCLI system. The BCNU@HCLI system was chosen to develop gel and cream semisolid dosage forms. The cream (C_BCNU@HCLI) released 29.6% of the loaded BCNU after 12 h in the Nylon synthetic membrane test and 31.6% in the collagen membrane test, higher by comparison to the gel. The new cream was evaluated in vivo in a chemically induced model of skin cancer in mice. Quantitative immunohistochemistry analysis showed stronger inhibition of B-cell lymphoma-2 (bcl-2) and cyclooxygenase 2 (cox-2) protein expression, known markers for cancer survival and aggressiveness, after the treatment with C_BCNU@HCLI by comparison to all the control treatment types, including an off-label magistral formula commercially available Carmustine cream as reference, bringing evidence that a clinoptilolite-based delivery systems could be used as a cancer drug carriers and controlled release systems (skin-targeted topical delivery systems). |
format | Online Article Text |
id | pubmed-6150160 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-61501602018-11-13 Inhibition of bcl-2 and cox-2 Protein Expression after Local Application of a New Carmustine-Loaded Clinoptilolite-Based Delivery System in a Chemically Induced Skin Cancer Model in Mice Ghiciuc, Cristina Mihaela Strat, Aurel Lulu Ochiuz, Lacramioara Lupusoru, Catalina Elena Ignat, Maria Vasile, Aurelia Grigorovici, Alexandru Stoleriu, Iulian Solcan, Carmen Molecules Article Our research has focused on in vitro and in vivo evaluations of a new Carmustine (BCNU)-loaded clinoptilolite-based delivery system. Two clinoptilolite ionic forms—hydrogen form (HCLI) and sodium form (NaCLI)—were prepared, allowing a loading degree of about 5–6 mg BCNU/g of zeolite matrix due to the dual porous feature of clinoptilolite. Clinoptilolite-based delivery systems released 35.23% of the load in 12 h for the BCNU@HCLI system and only 10.82% for the BCNU@NaCLI system. The BCNU@HCLI system was chosen to develop gel and cream semisolid dosage forms. The cream (C_BCNU@HCLI) released 29.6% of the loaded BCNU after 12 h in the Nylon synthetic membrane test and 31.6% in the collagen membrane test, higher by comparison to the gel. The new cream was evaluated in vivo in a chemically induced model of skin cancer in mice. Quantitative immunohistochemistry analysis showed stronger inhibition of B-cell lymphoma-2 (bcl-2) and cyclooxygenase 2 (cox-2) protein expression, known markers for cancer survival and aggressiveness, after the treatment with C_BCNU@HCLI by comparison to all the control treatment types, including an off-label magistral formula commercially available Carmustine cream as reference, bringing evidence that a clinoptilolite-based delivery systems could be used as a cancer drug carriers and controlled release systems (skin-targeted topical delivery systems). MDPI 2017-11-20 /pmc/articles/PMC6150160/ /pubmed/29156646 http://dx.doi.org/10.3390/molecules22112014 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ghiciuc, Cristina Mihaela Strat, Aurel Lulu Ochiuz, Lacramioara Lupusoru, Catalina Elena Ignat, Maria Vasile, Aurelia Grigorovici, Alexandru Stoleriu, Iulian Solcan, Carmen Inhibition of bcl-2 and cox-2 Protein Expression after Local Application of a New Carmustine-Loaded Clinoptilolite-Based Delivery System in a Chemically Induced Skin Cancer Model in Mice |
title | Inhibition of bcl-2 and cox-2 Protein Expression after Local Application of a New Carmustine-Loaded Clinoptilolite-Based Delivery System in a Chemically Induced Skin Cancer Model in Mice |
title_full | Inhibition of bcl-2 and cox-2 Protein Expression after Local Application of a New Carmustine-Loaded Clinoptilolite-Based Delivery System in a Chemically Induced Skin Cancer Model in Mice |
title_fullStr | Inhibition of bcl-2 and cox-2 Protein Expression after Local Application of a New Carmustine-Loaded Clinoptilolite-Based Delivery System in a Chemically Induced Skin Cancer Model in Mice |
title_full_unstemmed | Inhibition of bcl-2 and cox-2 Protein Expression after Local Application of a New Carmustine-Loaded Clinoptilolite-Based Delivery System in a Chemically Induced Skin Cancer Model in Mice |
title_short | Inhibition of bcl-2 and cox-2 Protein Expression after Local Application of a New Carmustine-Loaded Clinoptilolite-Based Delivery System in a Chemically Induced Skin Cancer Model in Mice |
title_sort | inhibition of bcl-2 and cox-2 protein expression after local application of a new carmustine-loaded clinoptilolite-based delivery system in a chemically induced skin cancer model in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6150160/ https://www.ncbi.nlm.nih.gov/pubmed/29156646 http://dx.doi.org/10.3390/molecules22112014 |
work_keys_str_mv | AT ghiciuccristinamihaela inhibitionofbcl2andcox2proteinexpressionafterlocalapplicationofanewcarmustineloadedclinoptilolitebaseddeliverysysteminachemicallyinducedskincancermodelinmice AT strataurellulu inhibitionofbcl2andcox2proteinexpressionafterlocalapplicationofanewcarmustineloadedclinoptilolitebaseddeliverysysteminachemicallyinducedskincancermodelinmice AT ochiuzlacramioara inhibitionofbcl2andcox2proteinexpressionafterlocalapplicationofanewcarmustineloadedclinoptilolitebaseddeliverysysteminachemicallyinducedskincancermodelinmice AT lupusorucatalinaelena inhibitionofbcl2andcox2proteinexpressionafterlocalapplicationofanewcarmustineloadedclinoptilolitebaseddeliverysysteminachemicallyinducedskincancermodelinmice AT ignatmaria inhibitionofbcl2andcox2proteinexpressionafterlocalapplicationofanewcarmustineloadedclinoptilolitebaseddeliverysysteminachemicallyinducedskincancermodelinmice AT vasileaurelia inhibitionofbcl2andcox2proteinexpressionafterlocalapplicationofanewcarmustineloadedclinoptilolitebaseddeliverysysteminachemicallyinducedskincancermodelinmice AT grigorovicialexandru inhibitionofbcl2andcox2proteinexpressionafterlocalapplicationofanewcarmustineloadedclinoptilolitebaseddeliverysysteminachemicallyinducedskincancermodelinmice AT stoleriuiulian inhibitionofbcl2andcox2proteinexpressionafterlocalapplicationofanewcarmustineloadedclinoptilolitebaseddeliverysysteminachemicallyinducedskincancermodelinmice AT solcancarmen inhibitionofbcl2andcox2proteinexpressionafterlocalapplicationofanewcarmustineloadedclinoptilolitebaseddeliverysysteminachemicallyinducedskincancermodelinmice |