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Applications of PNA-Based Artificial Restriction DNA Cutters

More than ten years ago, artificial restriction DNA cutters were developed by combining two pseudo-complementary peptide nucleic acid (pcPNA) strands with either Ce(IV)/EDTA or S1 nuclease. They have remarkably high site-specificity and can cut only one predetermined site in the human genome. In thi...

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Detalles Bibliográficos
Autores principales: Shigi, Narumi, Sumaoka, Jun, Komiyama, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151779/
https://www.ncbi.nlm.nih.gov/pubmed/28934140
http://dx.doi.org/10.3390/molecules22101586
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author Shigi, Narumi
Sumaoka, Jun
Komiyama, Makoto
author_facet Shigi, Narumi
Sumaoka, Jun
Komiyama, Makoto
author_sort Shigi, Narumi
collection PubMed
description More than ten years ago, artificial restriction DNA cutters were developed by combining two pseudo-complementary peptide nucleic acid (pcPNA) strands with either Ce(IV)/EDTA or S1 nuclease. They have remarkably high site-specificity and can cut only one predetermined site in the human genome. In this article, recent progress of these man-made tools have been reviewed. By cutting the human genome site-selectively, desired fragments can be clipped from either the termini of chromosomes (telomeres) or from the middle of genome. These fragments should provide important information on the biological functions of complicated genome system. DNA/RNA hybrid duplexes, which are formed in living cells, are also site-selectively hydrolyzed by these cutters. In order to further facilitate the applications of the artificial DNA cutters, various chemical modifications have been attempted. One of the most important successes is preparation of PNA derivatives which can form double-duplex invasion complex even under high salt conditions. This is important for in vivo applications, since the inside of living cells is abundant of metal ions. Furthermore, site-selective DNA cutters which require only one PNA strand, in place of a pair of pcPNA strands, are developed. This progress has opened a way to new fields of PNA-based biochemistry and biotechnology.
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spelling pubmed-61517792018-11-13 Applications of PNA-Based Artificial Restriction DNA Cutters Shigi, Narumi Sumaoka, Jun Komiyama, Makoto Molecules Review More than ten years ago, artificial restriction DNA cutters were developed by combining two pseudo-complementary peptide nucleic acid (pcPNA) strands with either Ce(IV)/EDTA or S1 nuclease. They have remarkably high site-specificity and can cut only one predetermined site in the human genome. In this article, recent progress of these man-made tools have been reviewed. By cutting the human genome site-selectively, desired fragments can be clipped from either the termini of chromosomes (telomeres) or from the middle of genome. These fragments should provide important information on the biological functions of complicated genome system. DNA/RNA hybrid duplexes, which are formed in living cells, are also site-selectively hydrolyzed by these cutters. In order to further facilitate the applications of the artificial DNA cutters, various chemical modifications have been attempted. One of the most important successes is preparation of PNA derivatives which can form double-duplex invasion complex even under high salt conditions. This is important for in vivo applications, since the inside of living cells is abundant of metal ions. Furthermore, site-selective DNA cutters which require only one PNA strand, in place of a pair of pcPNA strands, are developed. This progress has opened a way to new fields of PNA-based biochemistry and biotechnology. MDPI 2017-09-21 /pmc/articles/PMC6151779/ /pubmed/28934140 http://dx.doi.org/10.3390/molecules22101586 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Shigi, Narumi
Sumaoka, Jun
Komiyama, Makoto
Applications of PNA-Based Artificial Restriction DNA Cutters
title Applications of PNA-Based Artificial Restriction DNA Cutters
title_full Applications of PNA-Based Artificial Restriction DNA Cutters
title_fullStr Applications of PNA-Based Artificial Restriction DNA Cutters
title_full_unstemmed Applications of PNA-Based Artificial Restriction DNA Cutters
title_short Applications of PNA-Based Artificial Restriction DNA Cutters
title_sort applications of pna-based artificial restriction dna cutters
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151779/
https://www.ncbi.nlm.nih.gov/pubmed/28934140
http://dx.doi.org/10.3390/molecules22101586
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