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The Role of Solvent-Accessible Leu-208 of Cold-Active Pseudomonas fluorescens Strain AMS8 Lipase in Interfacial Activation, Substrate Accessibility and Low-Molecular Weight Esterification in the Presence of Toluene

The alkaline cold-active lipase from Pseudomonas fluorescens AMS8 undergoes major structural changes when reacted with hydrophobic organic solvents. In toluene, the AMS8 lipase catalytic region is exposed by the moving hydrophobic lid 2 (Glu-148 to Gly-167). Solvent-accessible surface area analysis...

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Detalles Bibliográficos
Autores principales:	Yaacob, Norhayati, Ahmad Kamarudin, Nor Hafizah, Leow, Adam Thean Chor, Salleh, Abu Bakar, Raja Abd Rahman, Raja Noor Zaliha, Mohamad Ali, Mohd Shukuri
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6152135/ https://www.ncbi.nlm.nih.gov/pubmed/28805665 http://dx.doi.org/10.3390/molecules22081312

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