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Synthesis of Aminoglycoside-2′-O-Methyl Oligoribonucleotide Fusions

Phosphoramidite building blocks of ribostamycin (3 and 4), that may be incorporated at any position of the oligonucleotide sequence, were synthesized. The building blocks, together with a previously described neomycin-modified solid support, were applied for the preparation of aminoglycoside-2′-O-me...

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Autores principales: Granqvist, Lotta, Kraszewski, Andrzej, Tähtinen, Ville, Virta, Pasi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154110/
https://www.ncbi.nlm.nih.gov/pubmed/28481305
http://dx.doi.org/10.3390/molecules22050760
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author Granqvist, Lotta
Kraszewski, Andrzej
Tähtinen, Ville
Virta, Pasi
author_facet Granqvist, Lotta
Kraszewski, Andrzej
Tähtinen, Ville
Virta, Pasi
author_sort Granqvist, Lotta
collection PubMed
description Phosphoramidite building blocks of ribostamycin (3 and 4), that may be incorporated at any position of the oligonucleotide sequence, were synthesized. The building blocks, together with a previously described neomycin-modified solid support, were applied for the preparation of aminoglycoside-2′-O-methyl oligoribonucleotide fusions. The fusions were used to clamp a single strand DNA sequence (a purine-rich strand of c-Myc promoter 1) to form triple helical 2′-O-methyl RNA/DNA-hybrid constructs. The potential of the aminoglycoside moieties to stabilize the triple helical constructs were studied by UV-melting profile analysis.
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spelling pubmed-61541102018-11-13 Synthesis of Aminoglycoside-2′-O-Methyl Oligoribonucleotide Fusions Granqvist, Lotta Kraszewski, Andrzej Tähtinen, Ville Virta, Pasi Molecules Article Phosphoramidite building blocks of ribostamycin (3 and 4), that may be incorporated at any position of the oligonucleotide sequence, were synthesized. The building blocks, together with a previously described neomycin-modified solid support, were applied for the preparation of aminoglycoside-2′-O-methyl oligoribonucleotide fusions. The fusions were used to clamp a single strand DNA sequence (a purine-rich strand of c-Myc promoter 1) to form triple helical 2′-O-methyl RNA/DNA-hybrid constructs. The potential of the aminoglycoside moieties to stabilize the triple helical constructs were studied by UV-melting profile analysis. MDPI 2017-05-08 /pmc/articles/PMC6154110/ /pubmed/28481305 http://dx.doi.org/10.3390/molecules22050760 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Granqvist, Lotta
Kraszewski, Andrzej
Tähtinen, Ville
Virta, Pasi
Synthesis of Aminoglycoside-2′-O-Methyl Oligoribonucleotide Fusions
title Synthesis of Aminoglycoside-2′-O-Methyl Oligoribonucleotide Fusions
title_full Synthesis of Aminoglycoside-2′-O-Methyl Oligoribonucleotide Fusions
title_fullStr Synthesis of Aminoglycoside-2′-O-Methyl Oligoribonucleotide Fusions
title_full_unstemmed Synthesis of Aminoglycoside-2′-O-Methyl Oligoribonucleotide Fusions
title_short Synthesis of Aminoglycoside-2′-O-Methyl Oligoribonucleotide Fusions
title_sort synthesis of aminoglycoside-2′-o-methyl oligoribonucleotide fusions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154110/
https://www.ncbi.nlm.nih.gov/pubmed/28481305
http://dx.doi.org/10.3390/molecules22050760
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