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The Influence of Chitosan Cross-linking on the Properties of Alginate Microparticles with Metformin Hydrochloride—In Vitro and In Vivo Evaluation

Sodium alginate is a polymer with unique ability to gel with different cross-linking agents in result of ionic and electrostatic interactions. Chitosan cross-linked alginate provides improvement of swelling and mucoadhesive properties and might be used to design sustained release dosage forms. There...

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Autores principales: Szekalska, Marta, Sosnowska, Katarzyna, Zakrzeska, Agnieszka, Kasacka, Irena, Lewandowska, Alicja, Winnicka, Katarzyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155789/
https://www.ncbi.nlm.nih.gov/pubmed/28117747
http://dx.doi.org/10.3390/molecules22010182
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author Szekalska, Marta
Sosnowska, Katarzyna
Zakrzeska, Agnieszka
Kasacka, Irena
Lewandowska, Alicja
Winnicka, Katarzyna
author_facet Szekalska, Marta
Sosnowska, Katarzyna
Zakrzeska, Agnieszka
Kasacka, Irena
Lewandowska, Alicja
Winnicka, Katarzyna
author_sort Szekalska, Marta
collection PubMed
description Sodium alginate is a polymer with unique ability to gel with different cross-linking agents in result of ionic and electrostatic interactions. Chitosan cross-linked alginate provides improvement of swelling and mucoadhesive properties and might be used to design sustained release dosage forms. Therefore, the aim of this research was to develop and evaluate possibility of preparing chitosan cross-linked alginate microparticles containing metformin hydrochloride by the spray-drying method. In addition, influence of cross-linking agent on the properties of microparticles was evaluated. Formulation of microparticles prepared by the spray drying of 2% alginate solution cross-linked by 0.1% chitosan was characterized by good mucoadhesive properties, high drug loading and prolonged metformin hydrochloride release. It was shown that designed microparticles reduced rat glucose blood level, delayed absorption of metformin hydrochloride and provided stable plasma drug concentration. Additionally, histopathological studies of pancreas, liver and kidneys indicated that all prepared microparticles improved degenerative changes in organs of diabetic rats. Moreover, no toxicity effect and no changes in rats behavior after oral administration of chitosan cross-linked alginate microparticles were noted.
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spelling pubmed-61557892018-11-13 The Influence of Chitosan Cross-linking on the Properties of Alginate Microparticles with Metformin Hydrochloride—In Vitro and In Vivo Evaluation Szekalska, Marta Sosnowska, Katarzyna Zakrzeska, Agnieszka Kasacka, Irena Lewandowska, Alicja Winnicka, Katarzyna Molecules Article Sodium alginate is a polymer with unique ability to gel with different cross-linking agents in result of ionic and electrostatic interactions. Chitosan cross-linked alginate provides improvement of swelling and mucoadhesive properties and might be used to design sustained release dosage forms. Therefore, the aim of this research was to develop and evaluate possibility of preparing chitosan cross-linked alginate microparticles containing metformin hydrochloride by the spray-drying method. In addition, influence of cross-linking agent on the properties of microparticles was evaluated. Formulation of microparticles prepared by the spray drying of 2% alginate solution cross-linked by 0.1% chitosan was characterized by good mucoadhesive properties, high drug loading and prolonged metformin hydrochloride release. It was shown that designed microparticles reduced rat glucose blood level, delayed absorption of metformin hydrochloride and provided stable plasma drug concentration. Additionally, histopathological studies of pancreas, liver and kidneys indicated that all prepared microparticles improved degenerative changes in organs of diabetic rats. Moreover, no toxicity effect and no changes in rats behavior after oral administration of chitosan cross-linked alginate microparticles were noted. MDPI 2017-01-22 /pmc/articles/PMC6155789/ /pubmed/28117747 http://dx.doi.org/10.3390/molecules22010182 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Szekalska, Marta
Sosnowska, Katarzyna
Zakrzeska, Agnieszka
Kasacka, Irena
Lewandowska, Alicja
Winnicka, Katarzyna
The Influence of Chitosan Cross-linking on the Properties of Alginate Microparticles with Metformin Hydrochloride—In Vitro and In Vivo Evaluation
title The Influence of Chitosan Cross-linking on the Properties of Alginate Microparticles with Metformin Hydrochloride—In Vitro and In Vivo Evaluation
title_full The Influence of Chitosan Cross-linking on the Properties of Alginate Microparticles with Metformin Hydrochloride—In Vitro and In Vivo Evaluation
title_fullStr The Influence of Chitosan Cross-linking on the Properties of Alginate Microparticles with Metformin Hydrochloride—In Vitro and In Vivo Evaluation
title_full_unstemmed The Influence of Chitosan Cross-linking on the Properties of Alginate Microparticles with Metformin Hydrochloride—In Vitro and In Vivo Evaluation
title_short The Influence of Chitosan Cross-linking on the Properties of Alginate Microparticles with Metformin Hydrochloride—In Vitro and In Vivo Evaluation
title_sort influence of chitosan cross-linking on the properties of alginate microparticles with metformin hydrochloride—in vitro and in vivo evaluation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6155789/
https://www.ncbi.nlm.nih.gov/pubmed/28117747
http://dx.doi.org/10.3390/molecules22010182
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