One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing

Recently developed transgenic techniques to explore and exploit the metabolic potential of microalgae present several drawbacks associated with the delivery of exogenous DNA into the cells and its subsequent integration at random sites within the genome. Here, we report a highly efficient multiplex...

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Detalles Bibliográficos
Autores principales: Serif, Manuel, Dubois, Gwendoline, Finoux, Anne-Laure, Teste, Marie-Ange, Jallet, Denis, Daboussi, Fayza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6156588/
https://www.ncbi.nlm.nih.gov/pubmed/30254261
http://dx.doi.org/10.1038/s41467-018-06378-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6156588/
https://www.ncbi.nlm.nih.gov/pubmed/30254261
http://dx.doi.org/10.1038/s41467-018-06378-9

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