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One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing
Recently developed transgenic techniques to explore and exploit the metabolic potential of microalgae present several drawbacks associated with the delivery of exogenous DNA into the cells and its subsequent integration at random sites within the genome. Here, we report a highly efficient multiplex...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6156588/ https://www.ncbi.nlm.nih.gov/pubmed/30254261 http://dx.doi.org/10.1038/s41467-018-06378-9 |