Machining protein microcrystals for structure determination by electron diffraction

We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure determination, bridging the crystal size gap between the nanometer scale of conventional electron diffracti...

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Detalles Bibliográficos
Autores principales: Duyvesteyn, Helen M. E., Kotecha, Abhay, Ginn, Helen M., Hecksel, Corey W., Beale, Emma V., de Haas, Felix, Evans, Gwyndaf, Zhang, Peijun, Chiu, Wah, Stuart, David I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6156647/
https://www.ncbi.nlm.nih.gov/pubmed/30171169
http://dx.doi.org/10.1073/pnas.1809978115

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6156647/
https://www.ncbi.nlm.nih.gov/pubmed/30171169
http://dx.doi.org/10.1073/pnas.1809978115

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