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Machining protein microcrystals for structure determination by electron diffraction

We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure determination, bridging the crystal size gap between the nanometer scale of conventional electron diffracti...

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Detalles Bibliográficos
Autores principales:	Duyvesteyn, Helen M. E., Kotecha, Abhay, Ginn, Helen M., Hecksel, Corey W., Beale, Emma V., de Haas, Felix, Evans, Gwyndaf, Zhang, Peijun, Chiu, Wah, Stuart, David I.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	National Academy of Sciences 2018
Materias:	Biological Sciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6156647/ https://www.ncbi.nlm.nih.gov/pubmed/30171169 http://dx.doi.org/10.1073/pnas.1809978115

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