TRUmiCount: correctly counting absolute numbers of molecules using unique molecular identifiers

MOTIVATION: Counting molecules using next-generation sequencing (NGS) suffers from PCR amplification bias, which reduces the accuracy of many quantitative NGS-based experimental methods such as RNA-Seq. This is true even if molecules are made distinguishable using unique molecular identifiers (UMIs)...

Descripción completa

Detalles Bibliográficos
Autores principales: Pflug, Florian G, von Haeseler, Arndt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Original Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157883/
https://www.ncbi.nlm.nih.gov/pubmed/29672674
http://dx.doi.org/10.1093/bioinformatics/bty283

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157883/
https://www.ncbi.nlm.nih.gov/pubmed/29672674
http://dx.doi.org/10.1093/bioinformatics/bty283

Ejemplares similares