3′-End labeling of nucleic acids by a polymerase ribozyme

A polymerase ribozyme can be used to label the 3′ end of RNA or DNA molecules by incorporating a variety of functionalized nucleotide analogs. Guided by a complementary template, the ribozyme adds a single nucleotide that may contain a fluorophore, biotin, azide or alkyne moiety, thus enabling the d...

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Detalles Bibliográficos
Autores principales: Samanta, Biswajit, Horning, David P, Joyce, Gerald F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158495/
https://www.ncbi.nlm.nih.gov/pubmed/29901762
http://dx.doi.org/10.1093/nar/gky513
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author Samanta, Biswajit
Horning, David P
Joyce, Gerald F
author_facet Samanta, Biswajit
Horning, David P
Joyce, Gerald F
author_sort Samanta, Biswajit
collection PubMed
description A polymerase ribozyme can be used to label the 3′ end of RNA or DNA molecules by incorporating a variety of functionalized nucleotide analogs. Guided by a complementary template, the ribozyme adds a single nucleotide that may contain a fluorophore, biotin, azide or alkyne moiety, thus enabling the detection and/or capture of selectively labeled materials. Employing a variety of commercially available nucleotide analogs, efficient labeling was demonstrated for model RNAs and DNAs, human microRNAs and natural tRNA.
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spelling pubmed-61584952018-10-02 3′-End labeling of nucleic acids by a polymerase ribozyme Samanta, Biswajit Horning, David P Joyce, Gerald F Nucleic Acids Res Methods Online A polymerase ribozyme can be used to label the 3′ end of RNA or DNA molecules by incorporating a variety of functionalized nucleotide analogs. Guided by a complementary template, the ribozyme adds a single nucleotide that may contain a fluorophore, biotin, azide or alkyne moiety, thus enabling the detection and/or capture of selectively labeled materials. Employing a variety of commercially available nucleotide analogs, efficient labeling was demonstrated for model RNAs and DNAs, human microRNAs and natural tRNA. Oxford University Press 2018-09-28 2018-06-13 /pmc/articles/PMC6158495/ /pubmed/29901762 http://dx.doi.org/10.1093/nar/gky513 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Methods Online
Samanta, Biswajit
Horning, David P
Joyce, Gerald F
3′-End labeling of nucleic acids by a polymerase ribozyme
title 3′-End labeling of nucleic acids by a polymerase ribozyme
title_full 3′-End labeling of nucleic acids by a polymerase ribozyme
title_fullStr 3′-End labeling of nucleic acids by a polymerase ribozyme
title_full_unstemmed 3′-End labeling of nucleic acids by a polymerase ribozyme
title_short 3′-End labeling of nucleic acids by a polymerase ribozyme
title_sort 3′-end labeling of nucleic acids by a polymerase ribozyme
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158495/
https://www.ncbi.nlm.nih.gov/pubmed/29901762
http://dx.doi.org/10.1093/nar/gky513
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