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Development of a novel and economical agar-based non-adherent three-dimensional culture method for enrichment of cancer stem-like cells

BACKGROUND: Non-adherent or ultra-low attachment three-dimensional (3D) culture, also called sphere formation assay, has been widely used to assess the malignant phenotype and stemness potential of transformed or cancer cells. This method is also popularly used to isolate the cancer stem-like cells...

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Detalles Bibliográficos
Autores principales: Gao, Weijie, Wu, Dinglan, Wang, Yuliang, Wang, Zhu, Zou, Chang, Dai, Yong, Ng, Chi-Fai, Teoh, Jeremy Yuen-Chun, Chan, Franky Leung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6158801/
https://www.ncbi.nlm.nih.gov/pubmed/30257704
http://dx.doi.org/10.1186/s13287-018-0987-x