NaOH low‐salt method for chloroplast isolation and highly pure cpDNA preparation from Aeluropus littoralis

PREMISE OF THE STUDY: High‐yield pure chloroplast DNA (cpDNA) is necessary for whole genome sequencing. Chloroplast extraction with traditional high‐salt methods causes damage to nuclei and destroys the integrity of organelles, which leads to high genomic contamination from the nucleus and mitochond...

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Detalles Bibliográficos
Autores principales: Nasiri, Najmeh, Nematzadeh, Ghorbanali, Shariati‐Joni, Vahid, Najafi‐Zarrini, Hamid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Protocol Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159644/
https://www.ncbi.nlm.nih.gov/pubmed/30276031
http://dx.doi.org/10.1002/aps3.1183

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6159644/
https://www.ncbi.nlm.nih.gov/pubmed/30276031
http://dx.doi.org/10.1002/aps3.1183

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