Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene

BACKGROUND: Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between efficiency, specificity and sensitivity in the ampl...

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Detalles Bibliográficos
Autores principales: Sambo, Francesco, Finotello, Francesca, Lavezzo, Enrico, Baruzzo, Giacomo, Masi, Giulia, Peta, Elektra, Falda, Marco, Toppo, Stefano, Barzon, Luisa, Di Camillo, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162885/
https://www.ncbi.nlm.nih.gov/pubmed/30268091
http://dx.doi.org/10.1186/s12859-018-2360-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162885/
https://www.ncbi.nlm.nih.gov/pubmed/30268091
http://dx.doi.org/10.1186/s12859-018-2360-6

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