CAV2 promotes the growth of renal cell carcinoma through the EGFR/PI3K/Akt pathway

BACKGROUND: Caveolin-2 (CAV2) is reported to have an important role in cancer. The following study investigated the expression and function of CAV2 in kidney cancer in vitro and in vivo. MATERIALS AND METHODS: Real-time PCR, immunohistochemistry and Western blotting analysis were used to determine C...

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Autores principales: Liu, Fu, Shangli, Zhi, Hu, Zhili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163017/
https://www.ncbi.nlm.nih.gov/pubmed/30288056
http://dx.doi.org/10.2147/OTT.S172803
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author Liu, Fu
Shangli, Zhi
Hu, Zhili
author_facet Liu, Fu
Shangli, Zhi
Hu, Zhili
author_sort Liu, Fu
collection PubMed
description BACKGROUND: Caveolin-2 (CAV2) is reported to have an important role in cancer. The following study investigated the expression and function of CAV2 in kidney cancer in vitro and in vivo. MATERIALS AND METHODS: Real-time PCR, immunohistochemistry and Western blotting analysis were used to determine CAV2, epidermal growth factor receptor (EGFR), phosphatidylinositol 3-kinase (PI3K) and protein kinase B (Akt) in kidney cancer cell line OS-RC-2 and clinical specimens. The role of CAV2 in maintaining kidney cancer malignant phenotype was examined by wound healing assay, Matrigel invasion assays and mouse orthotopic xenograft model. RESULTS: Higher expression of CAV2 was found in renal cell carcinoma tissue compared to normal tissue. Furthermore, increased expression of CAV2 was associated with cancer progression. Also, silencing of CAV2 inhibited the proliferation, migration and invasion, as well as the expression of EGFR, PI3K and p-Akt in OS-RC-2 cells in vitro, and OS-RC-2 xenograft growth in vivo. CONCLUSION: Our results revealed that CAV2 promotes the growth of renal cell carcinoma through EGFR/PI3K/Akt pathway.
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spelling pubmed-61630172018-10-04 CAV2 promotes the growth of renal cell carcinoma through the EGFR/PI3K/Akt pathway Liu, Fu Shangli, Zhi Hu, Zhili Onco Targets Ther Original Research BACKGROUND: Caveolin-2 (CAV2) is reported to have an important role in cancer. The following study investigated the expression and function of CAV2 in kidney cancer in vitro and in vivo. MATERIALS AND METHODS: Real-time PCR, immunohistochemistry and Western blotting analysis were used to determine CAV2, epidermal growth factor receptor (EGFR), phosphatidylinositol 3-kinase (PI3K) and protein kinase B (Akt) in kidney cancer cell line OS-RC-2 and clinical specimens. The role of CAV2 in maintaining kidney cancer malignant phenotype was examined by wound healing assay, Matrigel invasion assays and mouse orthotopic xenograft model. RESULTS: Higher expression of CAV2 was found in renal cell carcinoma tissue compared to normal tissue. Furthermore, increased expression of CAV2 was associated with cancer progression. Also, silencing of CAV2 inhibited the proliferation, migration and invasion, as well as the expression of EGFR, PI3K and p-Akt in OS-RC-2 cells in vitro, and OS-RC-2 xenograft growth in vivo. CONCLUSION: Our results revealed that CAV2 promotes the growth of renal cell carcinoma through EGFR/PI3K/Akt pathway. Dove Medical Press 2018-09-25 /pmc/articles/PMC6163017/ /pubmed/30288056 http://dx.doi.org/10.2147/OTT.S172803 Text en © 2018 Liu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Liu, Fu
Shangli, Zhi
Hu, Zhili
CAV2 promotes the growth of renal cell carcinoma through the EGFR/PI3K/Akt pathway
title CAV2 promotes the growth of renal cell carcinoma through the EGFR/PI3K/Akt pathway
title_full CAV2 promotes the growth of renal cell carcinoma through the EGFR/PI3K/Akt pathway
title_fullStr CAV2 promotes the growth of renal cell carcinoma through the EGFR/PI3K/Akt pathway
title_full_unstemmed CAV2 promotes the growth of renal cell carcinoma through the EGFR/PI3K/Akt pathway
title_short CAV2 promotes the growth of renal cell carcinoma through the EGFR/PI3K/Akt pathway
title_sort cav2 promotes the growth of renal cell carcinoma through the egfr/pi3k/akt pathway
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163017/
https://www.ncbi.nlm.nih.gov/pubmed/30288056
http://dx.doi.org/10.2147/OTT.S172803
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AT huzhili cav2promotesthegrowthofrenalcellcarcinomathroughtheegfrpi3kaktpathway

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