A One-Step Extraction and Luminescence Assay for Quantifying Glucose and ATP Levels in Cultured HepG2 Cells

A fluorescence-based enzymatic microplate intracellular glucose assay was designed and fully validated. The method was tested in a hepatocellular cancer cell line (HepG2). Our novel one-step extraction reagent gave stable cell lysates for glucose, adenosine triphosphate (ATP), and total protein dete...

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Detalles Bibliográficos
Autores principales: Csepregi, Rita, Temesfői, Viktória, Sali, Nikolett, Poór, Miklós, W. Needs, Paul, A. Kroon, Paul, Kőszegi, Tamás
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163413/
https://www.ncbi.nlm.nih.gov/pubmed/30205572
http://dx.doi.org/10.3390/ijms19092670

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163413/
https://www.ncbi.nlm.nih.gov/pubmed/30205572
http://dx.doi.org/10.3390/ijms19092670

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