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Automated Cell Treatment for Competence and Transformation of Escherichia coli in a High-Throughput Quasi-Turbidostat Using Microtiter Plates

Metabolic engineering and genome editing strategies often lead to large strain libraries of a bacterial host. Nevertheless, the generation of competent cells is the basis for transformation and subsequent screening of these strains. While preparation of competent cells is a standard procedure in fla...

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Detalles Bibliográficos
Autores principales: Hans, Sebastian, Gimpel, Matthias, Glauche, Florian, Neubauer, Peter, Cruz-Bournazou, Mariano Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163857/
https://www.ncbi.nlm.nih.gov/pubmed/29941834
http://dx.doi.org/10.3390/microorganisms6030060