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Automated Cell Treatment for Competence and Transformation of Escherichia coli in a High-Throughput Quasi-Turbidostat Using Microtiter Plates
Metabolic engineering and genome editing strategies often lead to large strain libraries of a bacterial host. Nevertheless, the generation of competent cells is the basis for transformation and subsequent screening of these strains. While preparation of competent cells is a standard procedure in fla...
Autores principales: | Hans, Sebastian, Gimpel, Matthias, Glauche, Florian, Neubauer, Peter, Cruz-Bournazou, Mariano Nicolas |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6163857/ https://www.ncbi.nlm.nih.gov/pubmed/29941834 http://dx.doi.org/10.3390/microorganisms6030060 |
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