Improving nanopore read accuracy with the R2C2 method enables the sequencing of highly multiplexed full-length single-cell cDNA

High-throughput short-read sequencing has revolutionized how transcriptomes are quantified and annotated. However, while Illumina short-read sequencers can be used to analyze entire transcriptomes down to the level of individual splicing events with great accuracy, they fall short of analyzing how t...

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Detalles Bibliográficos
Autores principales: Volden, Roger, Palmer, Theron, Byrne, Ashley, Cole, Charles, Schmitz, Robert J., Green, Richard E., Vollmers, Christopher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6166824/
https://www.ncbi.nlm.nih.gov/pubmed/30201725
http://dx.doi.org/10.1073/pnas.1806447115

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6166824/
https://www.ncbi.nlm.nih.gov/pubmed/30201725
http://dx.doi.org/10.1073/pnas.1806447115

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