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Improving nanopore read accuracy with the R2C2 method enables the sequencing of highly multiplexed full-length single-cell cDNA
High-throughput short-read sequencing has revolutionized how transcriptomes are quantified and annotated. However, while Illumina short-read sequencers can be used to analyze entire transcriptomes down to the level of individual splicing events with great accuracy, they fall short of analyzing how t...
Autores principales: | Volden, Roger, Palmer, Theron, Byrne, Ashley, Cole, Charles, Schmitz, Robert J., Green, Richard E., Vollmers, Christopher |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6166824/ https://www.ncbi.nlm.nih.gov/pubmed/30201725 http://dx.doi.org/10.1073/pnas.1806447115 |
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