Improvements in Bacterial Primers to Enhance Selective SSU rRNA Gene Amplification of Plant-associated Bacteria by Applying the LNA Oligonucleotide-PCR Clamping Technique

PCR clamping by locked nucleic acid (LNA) oligonucleotides is an effective technique for selectively amplifying the community SSU rRNA genes of plant–associated bacteria. However, the original primer set often shows low amplification efficiency. In order to improve this efficiency, new primers were...

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Detalles Bibliográficos
Autores principales: Ikenaga, Makoto, Katsuragi, Shohei, Handa, Yoshihiro, Katsumata, Hiroshi, Chishaki, Naoya, Kawauchi, Tomohiro, Sakai, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2018
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167120/
https://www.ncbi.nlm.nih.gov/pubmed/30146542
http://dx.doi.org/10.1264/jsme2.ME18071

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167120/
https://www.ncbi.nlm.nih.gov/pubmed/30146542
http://dx.doi.org/10.1264/jsme2.ME18071

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