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Influence of Fluorescent Protein Maturation on FRET Measurements in Living Cells

[Image: see text] Förster resonance energy transfer (FRET)-based sensors are a valuable tool to quantify cell biology, yet it remains necessary to identify and prevent potential artifacts in order to exploit their full potential. We show here that artifacts arising from slow donor mCerulean3 maturat...

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Detalles Bibliográficos
Autores principales: Liu, Boqun, Mavrova, Sara N., van den Berg, Jonas, Kristensen, Sebastian K., Mantovanelli, Luca, Veenhoff, Liesbeth M., Poolman, Bert, Boersma, Arnold J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6167724/
https://www.ncbi.nlm.nih.gov/pubmed/30168711
http://dx.doi.org/10.1021/acssensors.8b00473