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Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression
BACKGROUND: When developing CRISPR/Cas9 systems for crops, it is crucial to invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes, especially if the transformation system is difficult or time-consuming. Cotton is an important crop for the production of fiber, oil, and...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169012/ https://www.ncbi.nlm.nih.gov/pubmed/30305839 http://dx.doi.org/10.1186/s13007-018-0353-0 |