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Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos

Naive pluripotency exists in epiblast cells of mouse pre-implantation embryos. However, whether the naive pluripotency is transient or nonexistent in primate embryos remains unclear. Using RNA-seq in single blastomeres from 16-cell embryos through to hatched blastocysts of rhesus monkey, we construc...

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Autores principales: Liu, Denghui, Wang, Xinyi, He, Dajian, Sun, Chunli, He, Xiechao, Yan, Lanzhen, Li, Yizhou, Han, Jing-Dong J., Zheng, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169889/
https://www.ncbi.nlm.nih.gov/pubmed/30154223
http://dx.doi.org/10.1101/gr.233437.117
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author Liu, Denghui
Wang, Xinyi
He, Dajian
Sun, Chunli
He, Xiechao
Yan, Lanzhen
Li, Yizhou
Han, Jing-Dong J.
Zheng, Ping
author_facet Liu, Denghui
Wang, Xinyi
He, Dajian
Sun, Chunli
He, Xiechao
Yan, Lanzhen
Li, Yizhou
Han, Jing-Dong J.
Zheng, Ping
author_sort Liu, Denghui
collection PubMed
description Naive pluripotency exists in epiblast cells of mouse pre-implantation embryos. However, whether the naive pluripotency is transient or nonexistent in primate embryos remains unclear. Using RNA-seq in single blastomeres from 16-cell embryos through to hatched blastocysts of rhesus monkey, we constructed the lineage segregation roadmap in which the specification of trophectoderm, epiblast, and primitive endoderm is initiated simultaneously at the early blastocyst stage. Importantly, we uncovered the existence of distinct pluripotent states in monkey pre-implantation embryos. At the early- and middle-blastocyst stages, the epiblast cells have the transcriptome features of naive pluripotency, whereas they display a continuum of primed pluripotency characteristics at the late and hatched blastocyst stages. Moreover, we identified potential regulators that might play roles in the transition from naive to primed pluripotency. Thus, our study suggests the transient existence of naive pluripotency in primates and proposes an ideal time window for derivation of primate embryonic stem cells with naive pluripotency.
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spelling pubmed-61698892019-04-01 Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos Liu, Denghui Wang, Xinyi He, Dajian Sun, Chunli He, Xiechao Yan, Lanzhen Li, Yizhou Han, Jing-Dong J. Zheng, Ping Genome Res Research Naive pluripotency exists in epiblast cells of mouse pre-implantation embryos. However, whether the naive pluripotency is transient or nonexistent in primate embryos remains unclear. Using RNA-seq in single blastomeres from 16-cell embryos through to hatched blastocysts of rhesus monkey, we constructed the lineage segregation roadmap in which the specification of trophectoderm, epiblast, and primitive endoderm is initiated simultaneously at the early blastocyst stage. Importantly, we uncovered the existence of distinct pluripotent states in monkey pre-implantation embryos. At the early- and middle-blastocyst stages, the epiblast cells have the transcriptome features of naive pluripotency, whereas they display a continuum of primed pluripotency characteristics at the late and hatched blastocyst stages. Moreover, we identified potential regulators that might play roles in the transition from naive to primed pluripotency. Thus, our study suggests the transient existence of naive pluripotency in primates and proposes an ideal time window for derivation of primate embryonic stem cells with naive pluripotency. Cold Spring Harbor Laboratory Press 2018-10 /pmc/articles/PMC6169889/ /pubmed/30154223 http://dx.doi.org/10.1101/gr.233437.117 Text en © 2018 Liu et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research
Liu, Denghui
Wang, Xinyi
He, Dajian
Sun, Chunli
He, Xiechao
Yan, Lanzhen
Li, Yizhou
Han, Jing-Dong J.
Zheng, Ping
Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos
title Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos
title_full Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos
title_fullStr Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos
title_full_unstemmed Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos
title_short Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos
title_sort single-cell rna-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169889/
https://www.ncbi.nlm.nih.gov/pubmed/30154223
http://dx.doi.org/10.1101/gr.233437.117
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