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Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos
Naive pluripotency exists in epiblast cells of mouse pre-implantation embryos. However, whether the naive pluripotency is transient or nonexistent in primate embryos remains unclear. Using RNA-seq in single blastomeres from 16-cell embryos through to hatched blastocysts of rhesus monkey, we construc...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169889/ https://www.ncbi.nlm.nih.gov/pubmed/30154223 http://dx.doi.org/10.1101/gr.233437.117 |
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author | Liu, Denghui Wang, Xinyi He, Dajian Sun, Chunli He, Xiechao Yan, Lanzhen Li, Yizhou Han, Jing-Dong J. Zheng, Ping |
author_facet | Liu, Denghui Wang, Xinyi He, Dajian Sun, Chunli He, Xiechao Yan, Lanzhen Li, Yizhou Han, Jing-Dong J. Zheng, Ping |
author_sort | Liu, Denghui |
collection | PubMed |
description | Naive pluripotency exists in epiblast cells of mouse pre-implantation embryos. However, whether the naive pluripotency is transient or nonexistent in primate embryos remains unclear. Using RNA-seq in single blastomeres from 16-cell embryos through to hatched blastocysts of rhesus monkey, we constructed the lineage segregation roadmap in which the specification of trophectoderm, epiblast, and primitive endoderm is initiated simultaneously at the early blastocyst stage. Importantly, we uncovered the existence of distinct pluripotent states in monkey pre-implantation embryos. At the early- and middle-blastocyst stages, the epiblast cells have the transcriptome features of naive pluripotency, whereas they display a continuum of primed pluripotency characteristics at the late and hatched blastocyst stages. Moreover, we identified potential regulators that might play roles in the transition from naive to primed pluripotency. Thus, our study suggests the transient existence of naive pluripotency in primates and proposes an ideal time window for derivation of primate embryonic stem cells with naive pluripotency. |
format | Online Article Text |
id | pubmed-6169889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-61698892019-04-01 Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos Liu, Denghui Wang, Xinyi He, Dajian Sun, Chunli He, Xiechao Yan, Lanzhen Li, Yizhou Han, Jing-Dong J. Zheng, Ping Genome Res Research Naive pluripotency exists in epiblast cells of mouse pre-implantation embryos. However, whether the naive pluripotency is transient or nonexistent in primate embryos remains unclear. Using RNA-seq in single blastomeres from 16-cell embryos through to hatched blastocysts of rhesus monkey, we constructed the lineage segregation roadmap in which the specification of trophectoderm, epiblast, and primitive endoderm is initiated simultaneously at the early blastocyst stage. Importantly, we uncovered the existence of distinct pluripotent states in monkey pre-implantation embryos. At the early- and middle-blastocyst stages, the epiblast cells have the transcriptome features of naive pluripotency, whereas they display a continuum of primed pluripotency characteristics at the late and hatched blastocyst stages. Moreover, we identified potential regulators that might play roles in the transition from naive to primed pluripotency. Thus, our study suggests the transient existence of naive pluripotency in primates and proposes an ideal time window for derivation of primate embryonic stem cells with naive pluripotency. Cold Spring Harbor Laboratory Press 2018-10 /pmc/articles/PMC6169889/ /pubmed/30154223 http://dx.doi.org/10.1101/gr.233437.117 Text en © 2018 Liu et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Liu, Denghui Wang, Xinyi He, Dajian Sun, Chunli He, Xiechao Yan, Lanzhen Li, Yizhou Han, Jing-Dong J. Zheng, Ping Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos |
title | Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos |
title_full | Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos |
title_fullStr | Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos |
title_full_unstemmed | Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos |
title_short | Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos |
title_sort | single-cell rna-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6169889/ https://www.ncbi.nlm.nih.gov/pubmed/30154223 http://dx.doi.org/10.1101/gr.233437.117 |
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