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Sub-ångstrom cryo-EM structure of a prion protofibril reveals a polar clasp
The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method, MicroED, we reveal the sub-1Å resolution structure of a protofibril formed by a wild-type segment from the β2-α2 loop of the bank vole prion protein. Th...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6170007/ https://www.ncbi.nlm.nih.gov/pubmed/29335561 http://dx.doi.org/10.1038/s41594-017-0018-0 |
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| author | Gallagher-Jones, Marcus Glynn, Calina Boyer, David R. Martynowycz, Michael W. Hernandez, Evelyn Miao, Jennifer Zee, Chih-Te Novikova, Irina V. Goldschmidt, Lukasz McFarlane, Heather T. Helguera, Gustavo F. Evans, James E. Sawaya, Michael R. Cascio, Duilio Eisenberg, David Gonen, Tamir Rodriguez, Jose A. |
| author_facet | Gallagher-Jones, Marcus Glynn, Calina Boyer, David R. Martynowycz, Michael W. Hernandez, Evelyn Miao, Jennifer Zee, Chih-Te Novikova, Irina V. Goldschmidt, Lukasz McFarlane, Heather T. Helguera, Gustavo F. Evans, James E. Sawaya, Michael R. Cascio, Duilio Eisenberg, David Gonen, Tamir Rodriguez, Jose A. |
| author_sort | Gallagher-Jones, Marcus |
| collection | PubMed |
| description | The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method, MicroED, we reveal the sub-1Å resolution structure of a protofibril formed by a wild-type segment from the β2-α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we name ‘polar clasps’. |
| format | Online Article Text |
| id | pubmed-6170007 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2018 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-61700072018-10-03 Sub-ångstrom cryo-EM structure of a prion protofibril reveals a polar clasp Gallagher-Jones, Marcus Glynn, Calina Boyer, David R. Martynowycz, Michael W. Hernandez, Evelyn Miao, Jennifer Zee, Chih-Te Novikova, Irina V. Goldschmidt, Lukasz McFarlane, Heather T. Helguera, Gustavo F. Evans, James E. Sawaya, Michael R. Cascio, Duilio Eisenberg, David Gonen, Tamir Rodriguez, Jose A. Nat Struct Mol Biol Article The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method, MicroED, we reveal the sub-1Å resolution structure of a protofibril formed by a wild-type segment from the β2-α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we name ‘polar clasps’. 2018-01-15 2018-02 /pmc/articles/PMC6170007/ /pubmed/29335561 http://dx.doi.org/10.1038/s41594-017-0018-0 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Gallagher-Jones, Marcus Glynn, Calina Boyer, David R. Martynowycz, Michael W. Hernandez, Evelyn Miao, Jennifer Zee, Chih-Te Novikova, Irina V. Goldschmidt, Lukasz McFarlane, Heather T. Helguera, Gustavo F. Evans, James E. Sawaya, Michael R. Cascio, Duilio Eisenberg, David Gonen, Tamir Rodriguez, Jose A. Sub-ångstrom cryo-EM structure of a prion protofibril reveals a polar clasp |
| title | Sub-ångstrom cryo-EM structure of a prion protofibril reveals a polar clasp |
| title_full | Sub-ångstrom cryo-EM structure of a prion protofibril reveals a polar clasp |
| title_fullStr | Sub-ångstrom cryo-EM structure of a prion protofibril reveals a polar clasp |
| title_full_unstemmed | Sub-ångstrom cryo-EM structure of a prion protofibril reveals a polar clasp |
| title_short | Sub-ångstrom cryo-EM structure of a prion protofibril reveals a polar clasp |
| title_sort | sub-ångstrom cryo-em structure of a prion protofibril reveals a polar clasp |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6170007/ https://www.ncbi.nlm.nih.gov/pubmed/29335561 http://dx.doi.org/10.1038/s41594-017-0018-0 |
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