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Inhibition of the gyrA promoter by transcription-coupled DNA supercoiling in Escherichia coli
The E. coli gyrA promoter (P(gyrA)) is a DNA supercoiling sensitive promoter, stimulated by relaxation of DNA templates, and inhibited by (−) DNA supercoiling in bacteria. However, whether P(gyrA) can be inhibited by transient and localized transcription-coupled DNA supercoiling (TCDS) has not been...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6170449/ https://www.ncbi.nlm.nih.gov/pubmed/30282997 http://dx.doi.org/10.1038/s41598-018-33089-4 |
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author | Dages, Samantha Dages, Kelley Zhi, Xiaoduo Leng, Fenfei |
author_facet | Dages, Samantha Dages, Kelley Zhi, Xiaoduo Leng, Fenfei |
author_sort | Dages, Samantha |
collection | PubMed |
description | The E. coli gyrA promoter (P(gyrA)) is a DNA supercoiling sensitive promoter, stimulated by relaxation of DNA templates, and inhibited by (−) DNA supercoiling in bacteria. However, whether P(gyrA) can be inhibited by transient and localized transcription-coupled DNA supercoiling (TCDS) has not been fully examined. In this paper, using different DNA templates including the E. coli chromosome, we show that transient and localized TCDS strongly inhibits P(gyrA) in E. coli. This result can be explained by a twin-supercoiled domain model of transcription in which (+) and (−) supercoiled domains are generated around the transcribing RNA polymerase. We also find that fluoroquinolones, such as ciprofloxacin, can substantially increase the expression of the firefly luciferase under the control of the P(gyrA) coupled to a divergent IPTG-inducible promoter in the presence of IPTG. This stimulation of P(gyrA) by fluoroquinolones can be also explained by the twin-supercoiled domain model of transcription. This unique property of TCDS may be configured into a high throughput-screening (HTS) assay to identify antimicrobial compounds targeting bacterial DNA gyrase. |
format | Online Article Text |
id | pubmed-6170449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61704492018-10-05 Inhibition of the gyrA promoter by transcription-coupled DNA supercoiling in Escherichia coli Dages, Samantha Dages, Kelley Zhi, Xiaoduo Leng, Fenfei Sci Rep Article The E. coli gyrA promoter (P(gyrA)) is a DNA supercoiling sensitive promoter, stimulated by relaxation of DNA templates, and inhibited by (−) DNA supercoiling in bacteria. However, whether P(gyrA) can be inhibited by transient and localized transcription-coupled DNA supercoiling (TCDS) has not been fully examined. In this paper, using different DNA templates including the E. coli chromosome, we show that transient and localized TCDS strongly inhibits P(gyrA) in E. coli. This result can be explained by a twin-supercoiled domain model of transcription in which (+) and (−) supercoiled domains are generated around the transcribing RNA polymerase. We also find that fluoroquinolones, such as ciprofloxacin, can substantially increase the expression of the firefly luciferase under the control of the P(gyrA) coupled to a divergent IPTG-inducible promoter in the presence of IPTG. This stimulation of P(gyrA) by fluoroquinolones can be also explained by the twin-supercoiled domain model of transcription. This unique property of TCDS may be configured into a high throughput-screening (HTS) assay to identify antimicrobial compounds targeting bacterial DNA gyrase. Nature Publishing Group UK 2018-10-03 /pmc/articles/PMC6170449/ /pubmed/30282997 http://dx.doi.org/10.1038/s41598-018-33089-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Dages, Samantha Dages, Kelley Zhi, Xiaoduo Leng, Fenfei Inhibition of the gyrA promoter by transcription-coupled DNA supercoiling in Escherichia coli |
title | Inhibition of the gyrA promoter by transcription-coupled DNA supercoiling in Escherichia coli |
title_full | Inhibition of the gyrA promoter by transcription-coupled DNA supercoiling in Escherichia coli |
title_fullStr | Inhibition of the gyrA promoter by transcription-coupled DNA supercoiling in Escherichia coli |
title_full_unstemmed | Inhibition of the gyrA promoter by transcription-coupled DNA supercoiling in Escherichia coli |
title_short | Inhibition of the gyrA promoter by transcription-coupled DNA supercoiling in Escherichia coli |
title_sort | inhibition of the gyra promoter by transcription-coupled dna supercoiling in escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6170449/ https://www.ncbi.nlm.nih.gov/pubmed/30282997 http://dx.doi.org/10.1038/s41598-018-33089-4 |
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