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Two Engineered OBPs with opposite temperature-dependent affinities towards 1-aminoanthracene

Engineered odorant-binding proteins (OBPs) display tunable binding affinities triggered by temperature alterations. We designed and produced two engineered proteins based on OBP-I sequence: truncated OBP (tOBP) and OBP::GQ(20)::SP-DS3. The binding affinity of 1-aminoanthracene (1-AMA) to these prote...

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Detalles Bibliográficos
Autores principales: Gonçalves, Filipa, Castro, Tarsila G., Azoia, Nuno G., Ribeiro, Artur, Silva, Carla, Cavaco-Paulo, Artur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172251/
https://www.ncbi.nlm.nih.gov/pubmed/30287882
http://dx.doi.org/10.1038/s41598-018-33085-8