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Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology
The polymerase-associated factor 1 (Paf1) complex is a general transcription elongation factor of RNA polymerase II, which is composed of five core subunits, Paf1, Ctr9, Cdc73, Leo1, and Rtf1, and functions as a diverse platform that broadly affects gene expression genome-wide. In this study, we sol...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176576/ https://www.ncbi.nlm.nih.gov/pubmed/30224485 http://dx.doi.org/10.1073/pnas.1812256115 |
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author | Deng, Pujuan Zhou, Yuqiao Jiang, Junyi Li, Haojie Tian, Wei Cao, Yinghua Qin, Yan Kim, Jaehoon Roeder, Robert G. Patel, Dinshaw J. Wang, Zhanxin |
author_facet | Deng, Pujuan Zhou, Yuqiao Jiang, Junyi Li, Haojie Tian, Wei Cao, Yinghua Qin, Yan Kim, Jaehoon Roeder, Robert G. Patel, Dinshaw J. Wang, Zhanxin |
author_sort | Deng, Pujuan |
collection | PubMed |
description | The polymerase-associated factor 1 (Paf1) complex is a general transcription elongation factor of RNA polymerase II, which is composed of five core subunits, Paf1, Ctr9, Cdc73, Leo1, and Rtf1, and functions as a diverse platform that broadly affects gene expression genome-wide. In this study, we solved the 2.9-Å crystal structure of the core region composed of the Ctr9-Paf1-Cdc73 ternary complex from a thermophilic fungi, which provides a structural perspective of the molecular details of the organization and interactions involving the Paf1 subunits in the core complex. We find that Ctr9 is composed of 21 tetratricopeptide repeat (TPR) motifs that wrap three circular turns in a right-handed superhelical manner around the N-terminal region of an elongated single-polypeptide–chain scaffold of Paf1. The Cdc73 fragment is positioned within the surface groove of Ctr9, where it contacts mainly with Ctr9 and minimally with Paf1. We also identified that the Paf1 complex preferentially binds single-strand–containing DNAs. Our work provides structural insights into the overall architecture of the Paf1 complex and paves the road forward for understanding the molecular mechanisms of the Paf1 complex in transcriptional regulation. |
format | Online Article Text |
id | pubmed-6176576 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-61765762018-10-11 Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology Deng, Pujuan Zhou, Yuqiao Jiang, Junyi Li, Haojie Tian, Wei Cao, Yinghua Qin, Yan Kim, Jaehoon Roeder, Robert G. Patel, Dinshaw J. Wang, Zhanxin Proc Natl Acad Sci U S A Biological Sciences The polymerase-associated factor 1 (Paf1) complex is a general transcription elongation factor of RNA polymerase II, which is composed of five core subunits, Paf1, Ctr9, Cdc73, Leo1, and Rtf1, and functions as a diverse platform that broadly affects gene expression genome-wide. In this study, we solved the 2.9-Å crystal structure of the core region composed of the Ctr9-Paf1-Cdc73 ternary complex from a thermophilic fungi, which provides a structural perspective of the molecular details of the organization and interactions involving the Paf1 subunits in the core complex. We find that Ctr9 is composed of 21 tetratricopeptide repeat (TPR) motifs that wrap three circular turns in a right-handed superhelical manner around the N-terminal region of an elongated single-polypeptide–chain scaffold of Paf1. The Cdc73 fragment is positioned within the surface groove of Ctr9, where it contacts mainly with Ctr9 and minimally with Paf1. We also identified that the Paf1 complex preferentially binds single-strand–containing DNAs. Our work provides structural insights into the overall architecture of the Paf1 complex and paves the road forward for understanding the molecular mechanisms of the Paf1 complex in transcriptional regulation. National Academy of Sciences 2018-10-02 2018-09-17 /pmc/articles/PMC6176576/ /pubmed/30224485 http://dx.doi.org/10.1073/pnas.1812256115 Text en Copyright © 2018 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Deng, Pujuan Zhou, Yuqiao Jiang, Junyi Li, Haojie Tian, Wei Cao, Yinghua Qin, Yan Kim, Jaehoon Roeder, Robert G. Patel, Dinshaw J. Wang, Zhanxin Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology |
title | Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology |
title_full | Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology |
title_fullStr | Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology |
title_full_unstemmed | Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology |
title_short | Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology |
title_sort | transcriptional elongation factor paf1 core complex adopts a spirally wrapped solenoidal topology |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176576/ https://www.ncbi.nlm.nih.gov/pubmed/30224485 http://dx.doi.org/10.1073/pnas.1812256115 |
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