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Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology

The polymerase-associated factor 1 (Paf1) complex is a general transcription elongation factor of RNA polymerase II, which is composed of five core subunits, Paf1, Ctr9, Cdc73, Leo1, and Rtf1, and functions as a diverse platform that broadly affects gene expression genome-wide. In this study, we sol...

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Autores principales: Deng, Pujuan, Zhou, Yuqiao, Jiang, Junyi, Li, Haojie, Tian, Wei, Cao, Yinghua, Qin, Yan, Kim, Jaehoon, Roeder, Robert G., Patel, Dinshaw J., Wang, Zhanxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176576/
https://www.ncbi.nlm.nih.gov/pubmed/30224485
http://dx.doi.org/10.1073/pnas.1812256115
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author Deng, Pujuan
Zhou, Yuqiao
Jiang, Junyi
Li, Haojie
Tian, Wei
Cao, Yinghua
Qin, Yan
Kim, Jaehoon
Roeder, Robert G.
Patel, Dinshaw J.
Wang, Zhanxin
author_facet Deng, Pujuan
Zhou, Yuqiao
Jiang, Junyi
Li, Haojie
Tian, Wei
Cao, Yinghua
Qin, Yan
Kim, Jaehoon
Roeder, Robert G.
Patel, Dinshaw J.
Wang, Zhanxin
author_sort Deng, Pujuan
collection PubMed
description The polymerase-associated factor 1 (Paf1) complex is a general transcription elongation factor of RNA polymerase II, which is composed of five core subunits, Paf1, Ctr9, Cdc73, Leo1, and Rtf1, and functions as a diverse platform that broadly affects gene expression genome-wide. In this study, we solved the 2.9-Å crystal structure of the core region composed of the Ctr9-Paf1-Cdc73 ternary complex from a thermophilic fungi, which provides a structural perspective of the molecular details of the organization and interactions involving the Paf1 subunits in the core complex. We find that Ctr9 is composed of 21 tetratricopeptide repeat (TPR) motifs that wrap three circular turns in a right-handed superhelical manner around the N-terminal region of an elongated single-polypeptide–chain scaffold of Paf1. The Cdc73 fragment is positioned within the surface groove of Ctr9, where it contacts mainly with Ctr9 and minimally with Paf1. We also identified that the Paf1 complex preferentially binds single-strand–containing DNAs. Our work provides structural insights into the overall architecture of the Paf1 complex and paves the road forward for understanding the molecular mechanisms of the Paf1 complex in transcriptional regulation.
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spelling pubmed-61765762018-10-11 Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology Deng, Pujuan Zhou, Yuqiao Jiang, Junyi Li, Haojie Tian, Wei Cao, Yinghua Qin, Yan Kim, Jaehoon Roeder, Robert G. Patel, Dinshaw J. Wang, Zhanxin Proc Natl Acad Sci U S A Biological Sciences The polymerase-associated factor 1 (Paf1) complex is a general transcription elongation factor of RNA polymerase II, which is composed of five core subunits, Paf1, Ctr9, Cdc73, Leo1, and Rtf1, and functions as a diverse platform that broadly affects gene expression genome-wide. In this study, we solved the 2.9-Å crystal structure of the core region composed of the Ctr9-Paf1-Cdc73 ternary complex from a thermophilic fungi, which provides a structural perspective of the molecular details of the organization and interactions involving the Paf1 subunits in the core complex. We find that Ctr9 is composed of 21 tetratricopeptide repeat (TPR) motifs that wrap three circular turns in a right-handed superhelical manner around the N-terminal region of an elongated single-polypeptide–chain scaffold of Paf1. The Cdc73 fragment is positioned within the surface groove of Ctr9, where it contacts mainly with Ctr9 and minimally with Paf1. We also identified that the Paf1 complex preferentially binds single-strand–containing DNAs. Our work provides structural insights into the overall architecture of the Paf1 complex and paves the road forward for understanding the molecular mechanisms of the Paf1 complex in transcriptional regulation. National Academy of Sciences 2018-10-02 2018-09-17 /pmc/articles/PMC6176576/ /pubmed/30224485 http://dx.doi.org/10.1073/pnas.1812256115 Text en Copyright © 2018 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Deng, Pujuan
Zhou, Yuqiao
Jiang, Junyi
Li, Haojie
Tian, Wei
Cao, Yinghua
Qin, Yan
Kim, Jaehoon
Roeder, Robert G.
Patel, Dinshaw J.
Wang, Zhanxin
Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology
title Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology
title_full Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology
title_fullStr Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology
title_full_unstemmed Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology
title_short Transcriptional elongation factor Paf1 core complex adopts a spirally wrapped solenoidal topology
title_sort transcriptional elongation factor paf1 core complex adopts a spirally wrapped solenoidal topology
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6176576/
https://www.ncbi.nlm.nih.gov/pubmed/30224485
http://dx.doi.org/10.1073/pnas.1812256115
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