Viable Mice with Extensive Gene Humanization (25-kbp) Created Using Embryonic Stem Cell/Blastocyst and CRISPR/Zygote Injection Approaches

Here, we describe an expansion of the typical DNA size limitations associated with CRISPR knock-in technology, more specifically, the physical extent to which mouse genomic DNA can be replaced with donor (in this case, human) DNA at an orthologous locus by zygotic injection. Driving our efforts was...

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Detalles Bibliográficos
Autores principales: Leidy-Davis, Tiffany, Cheng, Kai, Goodwin, Leslie O., Morgan, Judith L., Juan, Wen Chun, Roca, Xavier, Ong, S. Tiong, Bergstrom, David E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6177426/
https://www.ncbi.nlm.nih.gov/pubmed/30301924
http://dx.doi.org/10.1038/s41598-018-33408-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6177426/
https://www.ncbi.nlm.nih.gov/pubmed/30301924
http://dx.doi.org/10.1038/s41598-018-33408-9

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