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Biomechanics show stem cell necessity for effective treatment of volumetric muscle loss using bioengineered constructs
Despite the regenerative capacity of muscle, tissue volume is not restored after volumetric muscle loss (VML), perhaps due to a loss-of-structural extracellular matrix. We recently demonstrated the structural and functional restoration of muscle tissue in a mouse model of VML using an engineered “bi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180087/ https://www.ncbi.nlm.nih.gov/pubmed/30323949 http://dx.doi.org/10.1038/s41536-018-0057-0 |
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author | Quarta, Marco Cromie Lear, Melinda J. Blonigan, Justin Paine, Patrick Chacon, Robert Rando, Thomas A. |
author_facet | Quarta, Marco Cromie Lear, Melinda J. Blonigan, Justin Paine, Patrick Chacon, Robert Rando, Thomas A. |
author_sort | Quarta, Marco |
collection | PubMed |
description | Despite the regenerative capacity of muscle, tissue volume is not restored after volumetric muscle loss (VML), perhaps due to a loss-of-structural extracellular matrix. We recently demonstrated the structural and functional restoration of muscle tissue in a mouse model of VML using an engineered “bioconstruct,” comprising an extracellular matrix scaffold (decellularized muscle), muscle stem cells (MuSCs), and muscle-resident cells (MRCs). To test the ability of the cell-based bioconstruct to restore whole-muscle biomechanics, we measured biomechanical parameters in uninjured muscles, muscles injured to produce VML lesions, and in muscles that were injured and then treated by implanting either the scaffolds alone or with bioconstructs containing the scaffolds, MuSCs, and MRCs. We measured the active and passive forces over a range of lengths, viscoelastic force relaxation, optimal length, and twitch dynamics. Injured muscles showed a narrowed length-tension curve or lower force over a narrower range of muscle lengths, and increased passive force. When treated with bioconstructs, but not with scaffolds alone, injured muscles showed active and passive length-tension relationships that were not different from uninjured muscles. Moreover, injured muscles treated with bioconstructs exhibited reduced fibrosis compared to injured muscles either untreated or treated with scaffolds alone. The cell-based bioconstruct is a promising treatment approach for future translational efforts to restore whole-muscle biomechanics in muscles with VML lesions. |
format | Online Article Text |
id | pubmed-6180087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61800872018-10-15 Biomechanics show stem cell necessity for effective treatment of volumetric muscle loss using bioengineered constructs Quarta, Marco Cromie Lear, Melinda J. Blonigan, Justin Paine, Patrick Chacon, Robert Rando, Thomas A. NPJ Regen Med Brief Communication Despite the regenerative capacity of muscle, tissue volume is not restored after volumetric muscle loss (VML), perhaps due to a loss-of-structural extracellular matrix. We recently demonstrated the structural and functional restoration of muscle tissue in a mouse model of VML using an engineered “bioconstruct,” comprising an extracellular matrix scaffold (decellularized muscle), muscle stem cells (MuSCs), and muscle-resident cells (MRCs). To test the ability of the cell-based bioconstruct to restore whole-muscle biomechanics, we measured biomechanical parameters in uninjured muscles, muscles injured to produce VML lesions, and in muscles that were injured and then treated by implanting either the scaffolds alone or with bioconstructs containing the scaffolds, MuSCs, and MRCs. We measured the active and passive forces over a range of lengths, viscoelastic force relaxation, optimal length, and twitch dynamics. Injured muscles showed a narrowed length-tension curve or lower force over a narrower range of muscle lengths, and increased passive force. When treated with bioconstructs, but not with scaffolds alone, injured muscles showed active and passive length-tension relationships that were not different from uninjured muscles. Moreover, injured muscles treated with bioconstructs exhibited reduced fibrosis compared to injured muscles either untreated or treated with scaffolds alone. The cell-based bioconstruct is a promising treatment approach for future translational efforts to restore whole-muscle biomechanics in muscles with VML lesions. Nature Publishing Group UK 2018-10-10 /pmc/articles/PMC6180087/ /pubmed/30323949 http://dx.doi.org/10.1038/s41536-018-0057-0 Text en © This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Brief Communication Quarta, Marco Cromie Lear, Melinda J. Blonigan, Justin Paine, Patrick Chacon, Robert Rando, Thomas A. Biomechanics show stem cell necessity for effective treatment of volumetric muscle loss using bioengineered constructs |
title | Biomechanics show stem cell necessity for effective treatment of volumetric muscle loss using bioengineered constructs |
title_full | Biomechanics show stem cell necessity for effective treatment of volumetric muscle loss using bioengineered constructs |
title_fullStr | Biomechanics show stem cell necessity for effective treatment of volumetric muscle loss using bioengineered constructs |
title_full_unstemmed | Biomechanics show stem cell necessity for effective treatment of volumetric muscle loss using bioengineered constructs |
title_short | Biomechanics show stem cell necessity for effective treatment of volumetric muscle loss using bioengineered constructs |
title_sort | biomechanics show stem cell necessity for effective treatment of volumetric muscle loss using bioengineered constructs |
topic | Brief Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180087/ https://www.ncbi.nlm.nih.gov/pubmed/30323949 http://dx.doi.org/10.1038/s41536-018-0057-0 |
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