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A GFP-strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis

One of the major obstacles to obtaining a complete structural and functional understanding of proteins encoded by the Mycobacterium tuberculosis (Mtb) pathogen is due to significant difficulties in producing recombinant mycobacterial proteins. Recent advances that have utilised the closely related M...

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Detalles Bibliográficos
Autores principales: Radhakrishnan, Anjana, Furze, Christopher M., Ahangar, Mohd Syed, Fullam, Elizabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180428/
https://www.ncbi.nlm.nih.gov/pubmed/30319771
http://dx.doi.org/10.1039/c8ra06237d