An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities

Base editor (BE) technology, which uses CRISPR-Cas9 to direct cytidine deaminase enzymatic activity to specific genomic loci, enables the highly efficient introduction of precise cytidine-to-thymidine DNA alterations(1–6). However, existing BEs create unwanted C to T alterations when more than one C...

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Detalles Bibliográficos
Autores principales: Gehrke, Jason M., Cervantes, Oliver, Clement, M. Kendell, Wu, Yuxuan, Zeng, Jing, Bauer, Daniel E., Pinello, Luca, Joung, J. Keith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6181770/
https://www.ncbi.nlm.nih.gov/pubmed/30059493
http://dx.doi.org/10.1038/nbt.4199

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6181770/
https://www.ncbi.nlm.nih.gov/pubmed/30059493
http://dx.doi.org/10.1038/nbt.4199

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