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An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities

Base editor (BE) technology, which uses CRISPR-Cas9 to direct cytidine deaminase enzymatic activity to specific genomic loci, enables the highly efficient introduction of precise cytidine-to-thymidine DNA alterations(1–6). However, existing BEs create unwanted C to T alterations when more than one C...

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Detalles Bibliográficos
Autores principales:	Gehrke, Jason M., Cervantes, Oliver, Clement, M. Kendell, Wu, Yuxuan, Zeng, Jing, Bauer, Daniel E., Pinello, Luca, Joung, J. Keith
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6181770/ https://www.ncbi.nlm.nih.gov/pubmed/30059493 http://dx.doi.org/10.1038/nbt.4199

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