High fidelity CRISPR/Cas9 increases precise monoallelic and biallelic editing events in primordial germ cells

Primordial germ cells (PGCs), the embryonic precursors of the sperm and egg, are used for the introduction of genetic modifications into avian genome. Introduction of small defined sequences using genome editing has not been demonstrated in bird species. Here, we compared oligonucleotide-mediated HD...

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Detalles Bibliográficos
Autores principales: Idoko-Akoh, Alewo, Taylor, Lorna, Sang, Helen M., McGrew, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6181960/
https://www.ncbi.nlm.nih.gov/pubmed/30310080
http://dx.doi.org/10.1038/s41598-018-33244-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6181960/
https://www.ncbi.nlm.nih.gov/pubmed/30310080
http://dx.doi.org/10.1038/s41598-018-33244-x

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