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Identification and characterization of a novel alkalistable and salt‐tolerant esterase from the deep‐sea hydrothermal vent of the East Pacific Rise
A novel esterase gene selected from metagenomic sequences of deep‐sea hydrothermal vents was successfully expressed in Escherichia coli. The recombinant protein (est‐OKK), which belongs to the lipolytic enzyme family V, exhibited high activity toward pNP‐esters with short acyl chains and especially...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182558/ https://www.ncbi.nlm.nih.gov/pubmed/29504251 http://dx.doi.org/10.1002/mbo3.601 |