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How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions

Most mammalian tissue cells experience oxygen partial pressures in vivo equivalent to 1–6% O(2) (i.e., physioxia). In standard cell culture, however, headspace O(2) levels are usually not actively regulated and under these conditions are ~18%. This drives hyperoxia in cell culture media that can aff...

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Detalles Bibliográficos
Autores principales: Stuart, Jeffrey A., Fonseca, Joao, Moradi, Fereshteh, Cunningham, Cassandra, Seliman, Bishoy, Worsfold, Cydney R., Dolan, Sarah, Abando, John, Maddalena, Lucas A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186316/
https://www.ncbi.nlm.nih.gov/pubmed/30363917
http://dx.doi.org/10.1155/2018/8238459