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How Supraphysiological Oxygen Levels in Standard Cell Culture Affect Oxygen-Consuming Reactions
Most mammalian tissue cells experience oxygen partial pressures in vivo equivalent to 1–6% O(2) (i.e., physioxia). In standard cell culture, however, headspace O(2) levels are usually not actively regulated and under these conditions are ~18%. This drives hyperoxia in cell culture media that can aff...
Autores principales: | Stuart, Jeffrey A., Fonseca, Joao, Moradi, Fereshteh, Cunningham, Cassandra, Seliman, Bishoy, Worsfold, Cydney R., Dolan, Sarah, Abando, John, Maddalena, Lucas A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186316/ https://www.ncbi.nlm.nih.gov/pubmed/30363917 http://dx.doi.org/10.1155/2018/8238459 |
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