Cargando…

A Horizontal Magnetic Tweezers and Its Use for Studying Single DNA Molecules

We report the development of a magnetic tweezers that can be used to micromanipulate single DNA molecules by applying picoNewton (pN)-scale forces in the horizontal plane. The resulting force–extension data from our experiments show high-resolution detection of changes in the DNA tether’s extension:...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fabian, Roberto, Tyson, Christopher, Tuma, Pamela L., Pegg, Ian, Sarkar, Abhijit
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187538/ https://www.ncbi.nlm.nih.gov/pubmed/30424121 http://dx.doi.org/10.3390/mi9040188

Ejemplares similares

A Horizontal Magnetic Tweezers for Studying Single DNA Molecules and DNA-Binding Proteins
por: Fabian, Roberto, et al.
Publicado: (2021)

Micromechanical Study of Hyperacetylated Nucleosomes Using Single Molecule Transverse Magnetic Tweezers
por: Gaire, Santosh, et al.
Publicado: (2023)

Single-molecule multiplexed profiling of protein–DNA complexes using magnetic tweezers
por: Liang, Lin, et al.
Publicado: (2021)

Skewed Brownian Fluctuations in Single-Molecule Magnetic Tweezers
por: Burnham, Daniel R., et al.
Publicado: (2014)

A high-resolution magnetic tweezer for single-molecule measurements
por: Kim, Kipom, et al.
Publicado: (2009)

Dissection of structural dynamics of chromatin fibers by single-molecule magnetic tweezers
por: Xiao, Xue, et al.
Publicado: (2018)

NAP1-Assisted Nucleosome Assembly on DNA Measured in Real Time by Single-Molecule Magnetic Tweezers
por: Vlijm, Rifka, et al.
Publicado: (2012)

Scanning a DNA Molecule for Bound Proteins Using Hybrid Magnetic and Optical Tweezers
por: van Loenhout, Marijn T. J., et al.
Publicado: (2013)

Disturbance-free rapid solution exchange for magnetic tweezers single-molecule studies
por: Le, Shimin, et al.
Publicado: (2015)

Torsional sensing of small-molecule binding using magnetic tweezers
por: Lipfert, Jan, et al.
Publicado: (2010)

Magnetic Forces and DNA Mechanics in Multiplexed Magnetic Tweezers
por: De Vlaminck, Iwijn, et al.
Publicado: (2012)

Multiplex flow magnetic tweezers reveal rare enzymatic events with single molecule precision
por: Agarwal, Rohit, et al.
Publicado: (2020)

High-Resolution Optical Tweezers for Single-Molecule Manipulation
por: Zhang, Xinming, et al.
Publicado: (2013)

Magnetic tweezers measurements of the nanomechanical properties of DNA in the presence of drugs
por: Salerno, Domenico, et al.
Publicado: (2010)

Characterizing single-molecule dynamics of viral RNA-dependent RNA polymerases with multiplexed magnetic tweezers
por: Kuijpers, Louis, et al.
Publicado: (2022)

Optical tweezer and TIRF microscopy for single molecule manipulation of RNA/DNA nanostructures including their rubbery property and single molecule counting
por: Ghimire, Chiran, et al.
Publicado: (2021)

Measurement of the magnetic moment of single Magnetospirillum gryphiswaldense cells by magnetic tweezers
por: Zahn, C., et al.
Publicado: (2017)

Probing DNA Helicase Kinetics with Temperature‐Controlled Magnetic Tweezers
por: Gollnick, Benjamin, et al.
Publicado: (2014)

Magnetic Tweezers for the Measurement of Twist and Torque
por: Lipfert, Jan, et al.
Publicado: (2014)

A Polypeptide-DNA Hybrid with Selective Linking Capability Applied to Single Molecule Nano-Mechanical Measurements Using Optical Tweezers
por: Moayed, Fatemeh, et al.
Publicado: (2013)

Determining Trap Compliances, Microsphere Size Variations, and Response Linearities in Single DNA Molecule Elasticity Measurements with Optical Tweezers
por: Mo, Youbin, et al.
Publicado: (2021)

Digital holography-based 3D particle localization for single-molecule tweezer techniques
por: Flewellen, James L., et al.
Publicado: (2022)

Single-Molecule Optical Tweezers As a Tool for Delineating the Mechanisms of Protein-Processing Mechanoenzymes
por: Mukherjee, Soham, et al.
Publicado: (2022)

Probing the salt dependence of the torsional stiffness of DNA by multiplexed magnetic torque tweezers
por: Kriegel, Franziska, et al.
Publicado: (2017)

Single Cell Isolation Using Optical Tweezers
por: Keloth, Anusha, et al.
Publicado: (2018)

Complexin-1 regulated assembly of single neuronal SNARE complex revealed by single-molecule optical tweezers
por: Hao, Tongrui, et al.
Publicado: (2023)

5-Methyl-cytosine stabilizes DNA but hinders DNA hybridization revealed by magnetic tweezers and simulations
por: Zhao, Xiao-Cong, et al.
Publicado: (2022)

Thermophoretic tweezers for single nanoparticle manipulation
por: Stergar, Jošt, et al.
Publicado: (2020)

Magnetic tweezers measurements of the nanomechanical stability of DNA against denaturation at various conditions of pH and ionic strength
por: Tempestini, Alessia, et al.
Publicado: (2013)

Determining the effects of DNA sequence on Hel308 helicase translocation along single-stranded DNA using nanopore tweezers
por: Craig, Jonathan M, et al.
Publicado: (2019)

Binding mechanism of anti-cancer chemotherapeutic drug mitoxantrone to DNA characterized by magnetic tweezers
por: Kreft, Dennis, et al.
Publicado: (2018)

Invincible DNA tethers: covalent DNA anchoring for enhanced temporal and force stability in magnetic tweezers experiments
por: Janissen, Richard, et al.
Publicado: (2014)

Mechanistic characterization of the DEAD-box RNA helicase Ded1 from yeast as revealed by a novel technique using single-molecule magnetic tweezers
por: Raj, Saurabh, et al.
Publicado: (2019)

Correction-free force calibration for magnetic tweezers experiments
por: Ostrofet, Eugen, et al.
Publicado: (2018)

Correction to: Binding mechanism of anti-cancer chemotherapeutic drug mitoxantrone to DNA characterized by magnetic tweezers
por: Kreft, Dennis, et al.
Publicado: (2019)

Submicrometer elasticity of double-stranded DNA revealed by precision force-extension measurements with magnetic tweezers
por: Shon, Min Ju, et al.
Publicado: (2019)

Applying torque to the Escherichia coli flagellar motor using magnetic tweezers
por: van Oene, Maarten M., et al.
Publicado: (2017)

Copper-free click chemistry for attachment of biomolecules in magnetic tweezers
por: Eeftens, Jorine M., et al.
Publicado: (2015)

Force determination in lateral magnetic tweezers combined with TIRF microscopy
por: Madariaga-Marcos, J., et al.
Publicado: (2018)

Mechanical unfolding of human telomere G-quadruplex DNA probed by integrated fluorescence and magnetic tweezers spectroscopy
por: Long, Xi, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_d55r87j5d6gj6c061b7h547q98