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Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing

Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out of 8,708 barcodes (4,354 s...

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Detalles Bibliográficos
Autores principales: Lee, Minho, Choi, Shin-Jung, Han, Sangjo, Nam, Miyoung, Kim, Dongsup, Kim, Dong-Uk, Hoe, Kwang-Lae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korea Genome Organization 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187811/
https://www.ncbi.nlm.nih.gov/pubmed/30304922
http://dx.doi.org/10.5808/GI.2018.16.2.22