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Comparative Analysis of B-Cell Receptor Repertoires Induced by Live Yellow Fever Vaccine in Young and Middle-Age Donors
Age-related changes can significantly alter the state of adaptive immune system and often lead to attenuated response to novel pathogens and vaccination. In present study we employed 5′RACE UMI-based full length and nearly error-free immunoglobulin profiling to compare plasma cell antibody repertoir...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189279/ https://www.ncbi.nlm.nih.gov/pubmed/30356675 http://dx.doi.org/10.3389/fimmu.2018.02309 |
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author | Davydov, Alexey N. Obraztsova, Anna S. Lebedin, Mikhail Y. Turchaninova, Maria A. Staroverov, Dmitriy B. Merzlyak, Ekaterina M. Sharonov, George V. Kladova, Olga Shugay, Mikhail Britanova, Olga V. Chudakov, Dmitriy M. |
author_facet | Davydov, Alexey N. Obraztsova, Anna S. Lebedin, Mikhail Y. Turchaninova, Maria A. Staroverov, Dmitriy B. Merzlyak, Ekaterina M. Sharonov, George V. Kladova, Olga Shugay, Mikhail Britanova, Olga V. Chudakov, Dmitriy M. |
author_sort | Davydov, Alexey N. |
collection | PubMed |
description | Age-related changes can significantly alter the state of adaptive immune system and often lead to attenuated response to novel pathogens and vaccination. In present study we employed 5′RACE UMI-based full length and nearly error-free immunoglobulin profiling to compare plasma cell antibody repertoires in young (19–26 years) and middle-age (45–58 years) individuals vaccinated with a live yellow fever vaccine, modeling a newly encountered pathogen. Our analysis has revealed age-related differences in the responding antibody repertoire ranging from distinct IGH CDR3 repertoire properties to differences in somatic hypermutation intensity and efficiency and antibody lineage tree structure. Overall, our findings suggest that younger individuals respond with a more diverse antibody repertoire and employ a more efficient somatic hypermutation process than elder individuals in response to a newly encountered pathogen. |
format | Online Article Text |
id | pubmed-6189279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61892792018-10-23 Comparative Analysis of B-Cell Receptor Repertoires Induced by Live Yellow Fever Vaccine in Young and Middle-Age Donors Davydov, Alexey N. Obraztsova, Anna S. Lebedin, Mikhail Y. Turchaninova, Maria A. Staroverov, Dmitriy B. Merzlyak, Ekaterina M. Sharonov, George V. Kladova, Olga Shugay, Mikhail Britanova, Olga V. Chudakov, Dmitriy M. Front Immunol Immunology Age-related changes can significantly alter the state of adaptive immune system and often lead to attenuated response to novel pathogens and vaccination. In present study we employed 5′RACE UMI-based full length and nearly error-free immunoglobulin profiling to compare plasma cell antibody repertoires in young (19–26 years) and middle-age (45–58 years) individuals vaccinated with a live yellow fever vaccine, modeling a newly encountered pathogen. Our analysis has revealed age-related differences in the responding antibody repertoire ranging from distinct IGH CDR3 repertoire properties to differences in somatic hypermutation intensity and efficiency and antibody lineage tree structure. Overall, our findings suggest that younger individuals respond with a more diverse antibody repertoire and employ a more efficient somatic hypermutation process than elder individuals in response to a newly encountered pathogen. Frontiers Media S.A. 2018-10-09 /pmc/articles/PMC6189279/ /pubmed/30356675 http://dx.doi.org/10.3389/fimmu.2018.02309 Text en Copyright © 2018 Davydov, Obraztsova, Lebedin, Turchaninova, Staroverov, Merzlyak, Sharonov, Kladova, Shugay, Britanova and Chudakov. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Davydov, Alexey N. Obraztsova, Anna S. Lebedin, Mikhail Y. Turchaninova, Maria A. Staroverov, Dmitriy B. Merzlyak, Ekaterina M. Sharonov, George V. Kladova, Olga Shugay, Mikhail Britanova, Olga V. Chudakov, Dmitriy M. Comparative Analysis of B-Cell Receptor Repertoires Induced by Live Yellow Fever Vaccine in Young and Middle-Age Donors |
title | Comparative Analysis of B-Cell Receptor Repertoires Induced by Live Yellow Fever Vaccine in Young and Middle-Age Donors |
title_full | Comparative Analysis of B-Cell Receptor Repertoires Induced by Live Yellow Fever Vaccine in Young and Middle-Age Donors |
title_fullStr | Comparative Analysis of B-Cell Receptor Repertoires Induced by Live Yellow Fever Vaccine in Young and Middle-Age Donors |
title_full_unstemmed | Comparative Analysis of B-Cell Receptor Repertoires Induced by Live Yellow Fever Vaccine in Young and Middle-Age Donors |
title_short | Comparative Analysis of B-Cell Receptor Repertoires Induced by Live Yellow Fever Vaccine in Young and Middle-Age Donors |
title_sort | comparative analysis of b-cell receptor repertoires induced by live yellow fever vaccine in young and middle-age donors |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189279/ https://www.ncbi.nlm.nih.gov/pubmed/30356675 http://dx.doi.org/10.3389/fimmu.2018.02309 |
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