A Multiplex Genome Editing Method for Escherichia coli Based on CRISPR-Cas12a

Various methods for editing specific sites in the Escherichia coli chromosome are available, and gene-size (∼1 kb) integration into a single site or to introduce deletions, short insertions or point mutations into multiple sites can be conducted in a short period of time. However, a method for rapid...

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Detalles Bibliográficos
Autores principales: Ao, Xiang, Yao, Yi, Li, Tian, Yang, Ting-Ting, Dong, Xu, Zheng, Ze-Tong, Chen, Guo-Qiang, Wu, Qiong, Guo, Yingying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189296/
https://www.ncbi.nlm.nih.gov/pubmed/30356638
http://dx.doi.org/10.3389/fmicb.2018.02307

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189296/
https://www.ncbi.nlm.nih.gov/pubmed/30356638
http://dx.doi.org/10.3389/fmicb.2018.02307

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